α-1,3 gal independent recognition of porcine endothelial cells (PAEC) by human Natural Killer (NK) cells

S. Sheikh, R. S. Parhar, K. Collison, S. Al-Sedairy, David Stern, F. A. Al-Mohanna

Research output: Contribution to journalArticle

Abstract

In a recent study (Am. J. Pathology, 151, 111-120, 1997), we reported that naïve human neutrophils recognized PAECs independently of xenoreactive antibodies and enhanced subsequent killing of xenoendothelial cells by NK cells. In the present study, we examined the effects of either soluble α1,3 gal or anti α1,3 gal antibody (Dextra Labs Ltd., Reading, UK) on the resting Ca++ levels of either xeno or allo endothelial cells during NK cell (highly purified CD16+ CD56+ >95% by FACS) mediated Ca++ dependent activation and killing of PAEC (xeno) or allo human aortic endothelial cell (HAEC). Results showed that direct contact between NK cells (2×105) and PAECs caused a transient Ca++ rise in the xenoendothelial cells from a resting level of 70 ± 2.3 nMol/L (PAECs, mean ± SEM; n = 9) to 339 ± 21.8 nMol/L. In the presence of either soluble α1,3 gal or anti α1,3 gal antibody, NK cells raised intracellular free Ca++ from resting level 78 ± 3.8 nMol/L to 426 ± 4.5 nMol/L, n=8 and 74 ± 5 to 508 ± 105 nMol/L, n=6 respectively. NK cells at 2:1 effector: target ratio resulted in a significant (p<0.001) killing of PAECs as compared to HAECs. No significant (p>0.01) inhibitory effects were observed using either soluble α1,3 gal or anti α1,3 gal antibody during the killing assay. Low level of HAECs killing was observed by NK cells, but no significant change in the Ca++ homeostasis was observed. These results suggest that NK cells recognize xenoendothelial cells and this recognition moiety seems to be independent of α1,3 gal.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998
Externally publishedYes

Fingerprint

Endothelial cells
natural killer cells
Natural Killer Cells
endothelial cells
Swine
Endothelial Cells
swine
Antibodies
antibodies
Pathology
Assays
Chemical activation
Cells
Scanning electron microscopy
cells
direct contact
neutrophils
Reading
homeostasis
Neutrophils

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Sheikh, S., Parhar, R. S., Collison, K., Al-Sedairy, S., Stern, D., & Al-Mohanna, F. A. (1998). α-1,3 gal independent recognition of porcine endothelial cells (PAEC) by human Natural Killer (NK) cells. FASEB Journal, 12(5).

α-1,3 gal independent recognition of porcine endothelial cells (PAEC) by human Natural Killer (NK) cells. / Sheikh, S.; Parhar, R. S.; Collison, K.; Al-Sedairy, S.; Stern, David; Al-Mohanna, F. A.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

Sheikh, S, Parhar, RS, Collison, K, Al-Sedairy, S, Stern, D & Al-Mohanna, FA 1998, 'α-1,3 gal independent recognition of porcine endothelial cells (PAEC) by human Natural Killer (NK) cells', FASEB Journal, vol. 12, no. 5.
Sheikh, S. ; Parhar, R. S. ; Collison, K. ; Al-Sedairy, S. ; Stern, David ; Al-Mohanna, F. A. / α-1,3 gal independent recognition of porcine endothelial cells (PAEC) by human Natural Killer (NK) cells. In: FASEB Journal. 1998 ; Vol. 12, No. 5.
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