αvβ3 Integrin Receptor Targeting and Near-Infrared Imaging of Solid Tumors Using Surface-Modified Nanoliposomes

Hari R. Desu, Laura A. Thoma, George C. Wood

Research output: Contribution to journalArticle

Abstract

Purpose: Abundance of receptors on tumor vasculature presents a prominent target for theranostic applications. The alphavbeta3 integrin receptors expressed on vascular endothelial cells during angiogenesis were therefore considered targets for imaging. Non-invasive visualization of tumor growth and/or delivery systems can appreciate tumor localization and disposition kinetics of carriers, respectively. Herein, we report near-infrared fluorescence imaging (NIRFI) of solid tumors using targeted fluorescence nanoliposomes in vivo. Methods: Fluorescence nanoliposomes surface modified with cRGD-peptide were injected into CD1 athymic (nu/nu) mice bearing C6 glioblastoma xenografts (300 mm3). At different time points, mice were subjected to NIRFI for visualization of tumor xenografts and nanocarrier tracing in vivo. Results: NIRFI showed tumor localization of 1,1′-dioctadecyl-3,3,3′,3′-tetramethyl indotricarbocyanine iodide (DiR18)-incorporated-targeted liposomes with maximum tumor-to-tissue occurring at 24-h post-liposome administration. Interaction of integrin receptors with targeted liposomes had contributed to an intense NIRF signal. Molecular studies showed an elevated expression of alphavbeta3 integrin receptors in tumor xenografts. Conclusion: From the studies, it can be concluded that non-invasive localization of tumors and tracing of liposome carriers had been achieved using receptor targeting and NIRFI approaches.

Original languageEnglish (US)
JournalJournal of Pharmaceutical Innovation
Volume13
Issue number1
DOIs
StatePublished - Mar 1 2018

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Integrins
Optical Imaging
Liposomes
Neoplasms
Integrin alphaVbeta3
Heterografts
Fluorescence
Iodides
Glioblastoma
Endothelial Cells
Growth

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science
  • Drug Discovery

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αvβ3 Integrin Receptor Targeting and Near-Infrared Imaging of Solid Tumors Using Surface-Modified Nanoliposomes. / Desu, Hari R.; Thoma, Laura A.; Wood, George C.

In: Journal of Pharmaceutical Innovation, Vol. 13, No. 1, 01.03.2018.

Research output: Contribution to journalArticle

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