β2-Adrenergic receptor polymorphisms in African American children with status asthmaticus

Lama Elbahlawan, Saeed Binaei, Michael Christensen, Qing Zhang, Michael W. Quasney, Mary K. Dahmer

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: The β2-adrenergic receptor plays a central role in the bronchodilator response to β2-agonists in patients with asthma. Genetic polymorphisms within the gene coding for this receptor influence responsiveness of the receptor. A number of these polymorphisms differ in frequency in the African American and white populations. Objective: To determine the frequency of specific β2-adrenergic receptor polymorphisms in African American children with status asthmaticus and to examine whether a specific genotype is associated with the clinical response to therapy. Design: Cohort of African American children diagnosed with status asthmaticus. Setting: Tertiary care children's hospital. Patients: A total of 31 African American children with status asthmaticus. Intervention: Blood samples were obtained from children at admission. Genotypes were determined by polymerase chain reaction amplification and restriction enzyme digestion. Main Outcome Measures: The requirement for admission to the pediatric intensive care unit, need for mechanical ventilation, institution of various therapies, and length of stay. Results: The genotypes of the polymorphic sites at amino acid positions 16 and 27 in the β2-adrenergic receptor were determined. There were no significant differences between the various genotypes in the percentage of children requiring pediatric intensive care unit admission, mechanical ventilation, terbutaline treatment, or length of stay. However, in children heterozygous for Glu at position 27 of the β2-adrenergic receptor, the percentage of patients requiring aminophylline treatment, in addition to β2-agonist therapy, was significantly higher than that seen in patients homozygous for Gln at that position (5/10 [50%] vs. 1/21 [5%], respectively; p = .002). Conclusions: African American children with status asthmaticus who have the Gln/Glu genotype at amino acid position 27 of the β2-adrenergic receptor may benefit from aminophylline treatment in addition to β2-agonist therapy.

Original languageEnglish (US)
Pages (from-to)15-18
Number of pages4
JournalPediatric Critical Care Medicine
Volume7
Issue number1
DOIs
StatePublished - Jan 1 2006

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Status Asthmaticus
African Americans
Adrenergic Receptors
Genotype
Aminophylline
Pediatric Intensive Care Units
Artificial Respiration
Therapeutics
Length of Stay
Amino Acids
Terbutaline
Bronchodilator Agents
Genetic Polymorphisms
Tertiary Healthcare
Digestion
Asthma
Outcome Assessment (Health Care)
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health
  • Critical Care and Intensive Care Medicine

Cite this

β2-Adrenergic receptor polymorphisms in African American children with status asthmaticus. / Elbahlawan, Lama; Binaei, Saeed; Christensen, Michael; Zhang, Qing; Quasney, Michael W.; Dahmer, Mary K.

In: Pediatric Critical Care Medicine, Vol. 7, No. 1, 01.01.2006, p. 15-18.

Research output: Contribution to journalArticle

Elbahlawan, Lama ; Binaei, Saeed ; Christensen, Michael ; Zhang, Qing ; Quasney, Michael W. ; Dahmer, Mary K. / β2-Adrenergic receptor polymorphisms in African American children with status asthmaticus. In: Pediatric Critical Care Medicine. 2006 ; Vol. 7, No. 1. pp. 15-18.
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abstract = "Background: The β2-adrenergic receptor plays a central role in the bronchodilator response to β2-agonists in patients with asthma. Genetic polymorphisms within the gene coding for this receptor influence responsiveness of the receptor. A number of these polymorphisms differ in frequency in the African American and white populations. Objective: To determine the frequency of specific β2-adrenergic receptor polymorphisms in African American children with status asthmaticus and to examine whether a specific genotype is associated with the clinical response to therapy. Design: Cohort of African American children diagnosed with status asthmaticus. Setting: Tertiary care children's hospital. Patients: A total of 31 African American children with status asthmaticus. Intervention: Blood samples were obtained from children at admission. Genotypes were determined by polymerase chain reaction amplification and restriction enzyme digestion. Main Outcome Measures: The requirement for admission to the pediatric intensive care unit, need for mechanical ventilation, institution of various therapies, and length of stay. Results: The genotypes of the polymorphic sites at amino acid positions 16 and 27 in the β2-adrenergic receptor were determined. There were no significant differences between the various genotypes in the percentage of children requiring pediatric intensive care unit admission, mechanical ventilation, terbutaline treatment, or length of stay. However, in children heterozygous for Glu at position 27 of the β2-adrenergic receptor, the percentage of patients requiring aminophylline treatment, in addition to β2-agonist therapy, was significantly higher than that seen in patients homozygous for Gln at that position (5/10 [50{\%}] vs. 1/21 [5{\%}], respectively; p = .002). Conclusions: African American children with status asthmaticus who have the Gln/Glu genotype at amino acid position 27 of the β2-adrenergic receptor may benefit from aminophylline treatment in addition to β2-agonist therapy.",
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AB - Background: The β2-adrenergic receptor plays a central role in the bronchodilator response to β2-agonists in patients with asthma. Genetic polymorphisms within the gene coding for this receptor influence responsiveness of the receptor. A number of these polymorphisms differ in frequency in the African American and white populations. Objective: To determine the frequency of specific β2-adrenergic receptor polymorphisms in African American children with status asthmaticus and to examine whether a specific genotype is associated with the clinical response to therapy. Design: Cohort of African American children diagnosed with status asthmaticus. Setting: Tertiary care children's hospital. Patients: A total of 31 African American children with status asthmaticus. Intervention: Blood samples were obtained from children at admission. Genotypes were determined by polymerase chain reaction amplification and restriction enzyme digestion. Main Outcome Measures: The requirement for admission to the pediatric intensive care unit, need for mechanical ventilation, institution of various therapies, and length of stay. Results: The genotypes of the polymorphic sites at amino acid positions 16 and 27 in the β2-adrenergic receptor were determined. There were no significant differences between the various genotypes in the percentage of children requiring pediatric intensive care unit admission, mechanical ventilation, terbutaline treatment, or length of stay. However, in children heterozygous for Glu at position 27 of the β2-adrenergic receptor, the percentage of patients requiring aminophylline treatment, in addition to β2-agonist therapy, was significantly higher than that seen in patients homozygous for Gln at that position (5/10 [50%] vs. 1/21 [5%], respectively; p = .002). Conclusions: African American children with status asthmaticus who have the Gln/Glu genotype at amino acid position 27 of the β2-adrenergic receptor may benefit from aminophylline treatment in addition to β2-agonist therapy.

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