2-Chloroacetaldehyde and 2-chloroacetal are potent inhibitors of DNA synthesis in animal cells

Jagannadha C. Kandala, John E.K. Mrema, Anthony DeAngelo, F. Bernard Daniel, Ramareddy Guntaka

Research output: Contribution to journalArticle

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Abstract

The effect of 2-chloroacetaldehyde, CAA, a metabolite of vinyl chloride and 2-chloroacetal, CAC, an ethyl diester of chloroacetaldehyde, on DNA synthesis in animal cells has been investigated. Both compounds drastically inhibited DNA synthesis at 10 to 20 μM. The inhibitory effect of the chemicals appears to be directly on DNA synthesis rather than on the uptake of thymidine or the formation of nucleotides. Residual DNA made in the presence of CAA had an average chain length of 300 nucleotides compared to a length of several thousand nucleotides in the absence of CAA. Synchronization experiments revealed that the inhibitory effect is reversible if 2-chloroacetaldehyde is removed within two hours but not after longer exposures.

Original languageEnglish (US)
Pages (from-to)457-463
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume167
Issue number2
DOIs
StatePublished - Mar 16 1990

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Nucleic Acid Synthesis Inhibitors
Animals
Cells
Nucleotides
DNA
Vinyl Chloride
Metabolites
Chain length
Thymidine
Synchronization
2-chloroacetal
chloroacetaldehyde
Experiments

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

2-Chloroacetaldehyde and 2-chloroacetal are potent inhibitors of DNA synthesis in animal cells. / Kandala, Jagannadha C.; Mrema, John E.K.; DeAngelo, Anthony; Daniel, F. Bernard; Guntaka, Ramareddy.

In: Biochemical and Biophysical Research Communications, Vol. 167, No. 2, 16.03.1990, p. 457-463.

Research output: Contribution to journalArticle

Kandala, Jagannadha C. ; Mrema, John E.K. ; DeAngelo, Anthony ; Daniel, F. Bernard ; Guntaka, Ramareddy. / 2-Chloroacetaldehyde and 2-chloroacetal are potent inhibitors of DNA synthesis in animal cells. In: Biochemical and Biophysical Research Communications. 1990 ; Vol. 167, No. 2. pp. 457-463.
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