3-dimensional morphometric analysis of murine bladder development and dysmorphogenesis

Ashley Carpenter, Andrew Paulus, Melissa Robinson, Carlton M. Bates, Michael L. Robinson, David Hains, David Kline, Kirk M. Mchugh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Disorders of the urinary tract represent a major cause of morbidity and impaired quality of life. To better understand the morphological events responsible for normal urinary tract development, we performed 3-D reconstructive analysis of developing mouse bladders in control, mgb-/-, and Fgfr2 Mes-/- mice. Results: Detrusor smooth muscle differentiation initiated in the bladder dome and progressed caudally with the leading edge extending down the right posterior surface of the bladder. Gender-specific differences in detrusor smooth muscle development were observed during early embryonic development. Bladder trigone morphology transitioned from an isosceles to equilateral triangle during development due to the preferential lengthening of the urethra to ureter distance. The primary defect observed in mgb-/- bladders was a significant reduction in detrusor smooth muscle differentiation throughout development. Deviations from normal trigone morphology correlated best with VUR development in Fgfr2 Mes-/- mice, while alterations in intravesicular tunnel length did not. Conclusions: Multivariate morphometric analysis provides a powerful tool to quantify and assess urinary tract development.

Original languageEnglish (US)
Pages (from-to)522-533
Number of pages12
JournalDevelopmental Dynamics
Volume241
Issue number3
DOIs
StatePublished - Mar 1 2012

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Urinary Bladder
Urinary Tract
Smooth Muscle
Muscle Development
Urethra
Ureter
Embryonic Development
Multivariate Analysis
Quality of Life
Morbidity

All Science Journal Classification (ASJC) codes

  • Developmental Biology

Cite this

Carpenter, A., Paulus, A., Robinson, M., Bates, C. M., Robinson, M. L., Hains, D., ... Mchugh, K. M. (2012). 3-dimensional morphometric analysis of murine bladder development and dysmorphogenesis. Developmental Dynamics, 241(3), 522-533. https://doi.org/10.1002/dvdy.23744

3-dimensional morphometric analysis of murine bladder development and dysmorphogenesis. / Carpenter, Ashley; Paulus, Andrew; Robinson, Melissa; Bates, Carlton M.; Robinson, Michael L.; Hains, David; Kline, David; Mchugh, Kirk M.

In: Developmental Dynamics, Vol. 241, No. 3, 01.03.2012, p. 522-533.

Research output: Contribution to journalArticle

Carpenter, A, Paulus, A, Robinson, M, Bates, CM, Robinson, ML, Hains, D, Kline, D & Mchugh, KM 2012, '3-dimensional morphometric analysis of murine bladder development and dysmorphogenesis', Developmental Dynamics, vol. 241, no. 3, pp. 522-533. https://doi.org/10.1002/dvdy.23744
Carpenter, Ashley ; Paulus, Andrew ; Robinson, Melissa ; Bates, Carlton M. ; Robinson, Michael L. ; Hains, David ; Kline, David ; Mchugh, Kirk M. / 3-dimensional morphometric analysis of murine bladder development and dysmorphogenesis. In: Developmental Dynamics. 2012 ; Vol. 241, No. 3. pp. 522-533.
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