\4 integrin expression on in vitro and in vivo metastatic variants of lewis lung carcinoma

L. Cimino, D. Perrotti, R. Falcioni, Stephen Kennel, A. Sacchi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The expression of β4, α6, and β1 integrin subunits has been investigated on some in vitro and in vivo murine metastatic variants derived from Lewis lung carcinoma (3LL). By the use of monoclonal antibodies which recognizes different epitopes of α6, β1, and β4 subunits we demonstrate that α6 and β1 subunits are expressed in all metastatic variants of 3LL irrespective of their metastatic potential, whereas β4 subunit is expressed only in highly metastasizing cells of 3LL. Northern blots of different metastatic variants probed with β1 and β4 subunits demonstrate that a) significant amounts of β1 mRNA were detected in all metastatic variants of 3LL;b) mRNA corresponding to the described entire coding sequence of β4 subunit is expressed only on highly metastasizing cells of 3LL. We conclude that β4 subunit is specifically expressed in highly metastasizig cells of 3LL while is undetectable in lower metastasizing ones.

Original languageEnglish (US)
Pages (from-to)45-48
Number of pages4
JournalCytotechnology
Volume5
Issue number1 Supplement
DOIs
StatePublished - Feb 1 1991
Externally publishedYes

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Lewis Lung Carcinoma
Integrins
Epitopes
Messenger RNA
Monoclonal antibodies
Monoclonal Antibodies
Northern Blotting
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Cell Biology
  • Biotechnology

Cite this

\4 integrin expression on in vitro and in vivo metastatic variants of lewis lung carcinoma. / Cimino, L.; Perrotti, D.; Falcioni, R.; Kennel, Stephen; Sacchi, A.

In: Cytotechnology, Vol. 5, No. 1 Supplement, 01.02.1991, p. 45-48.

Research output: Contribution to journalArticle

Cimino, L. ; Perrotti, D. ; Falcioni, R. ; Kennel, Stephen ; Sacchi, A. / \4 integrin expression on in vitro and in vivo metastatic variants of lewis lung carcinoma. In: Cytotechnology. 1991 ; Vol. 5, No. 1 Supplement. pp. 45-48.
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