7S Domain Constitutes the Amino-Terminal End of Type IV Collagen

an Immunochemical Study

Saryu N. Dixit, Carlo L. Mainardi, Edwin H. Beachey, Andrew Kang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The collagenous material from bovine kidney cortex was prepared by pepsin digestion of cortex homogenates. The 7S collagen was isolated after bacterial collagenase digestion of collagenous basement membrane material and was further purified by molecular sieve and ion exchange chromatography. The antibodies were raised in rabbits against a purified 7S collagen preparation. The pepsinderived collagenous fragments α1(IV)130K, 95K and α2(IV)120K prepared from bovine cortex were tested for their cross-reactivity with the antiserum. The fragment α1(IV)130K was found to be significantly cross-reactive. The results show the presence of common structural antigenic determinants between 7S collagen and α1(IV)130K fragment, thus indicating that 7S collagen is located at the aminoterminal end of type IV collagen.

Original languageEnglish (US)
Pages (from-to)263-269
Number of pages7
JournalTopics in Catalysis
Volume3
Issue number3
DOIs
StatePublished - Jan 1 1983

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Collagen Type IV
Collagen
Digestion
Kidney Cortex
Pepsin A
Molecular sieves
Ion Exchange Chromatography
Collagenases
Chromatography
Basement Membrane
Antibodies
Immune Sera
Epitopes
Ion exchange
Rabbits

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Rheumatology

Cite this

7S Domain Constitutes the Amino-Terminal End of Type IV Collagen : an Immunochemical Study. / Dixit, Saryu N.; Mainardi, Carlo L.; Beachey, Edwin H.; Kang, Andrew.

In: Topics in Catalysis, Vol. 3, No. 3, 01.01.1983, p. 263-269.

Research output: Contribution to journalArticle

Dixit, Saryu N. ; Mainardi, Carlo L. ; Beachey, Edwin H. ; Kang, Andrew. / 7S Domain Constitutes the Amino-Terminal End of Type IV Collagen : an Immunochemical Study. In: Topics in Catalysis. 1983 ; Vol. 3, No. 3. pp. 263-269.
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