A chimeric plum pox virus shows reduced spread and cannot compete with its parental wild-type viruses in a mixed infection

Christof Dietrich, Qusai Al Abdallah, Lara Lintl, Agnes Pietruszka, Edgar Maiss

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The effect of a recombination event in the genomic 3′ end on the biological properties and competitiveness of plum pox virus (PPV) was investigated. Therefore, a fragment spanning the coat protein (CP) coding region and a part of the 3′ non-translated region of a non-aphid-transmissible strain of PPV (PPV-NAT) was replaced by the corresponding region of a PPV sour cherry isolate (PPV-SoC). The resulting chimera (PPV-NAT/SoC) caused severe symptoms in Nicotiana benthamiana, resembling those of PPV-NAT. In mixed infections with either of the parental viruses, the chimera PPV-NAT/SoC was less competitive. Labelling experiments with DsRed showed that PPV-NAT/SoC (PPV-NAT/SoC-red) moved more slowly from cell to cell than PPV-NAT (PPV-NAT-red). In mixed infections of PPV-NAT/SoC-red with a green fluorescent protein-expressing PPV-NAT (PPV-NAT-AgfpS), spatial separation of the viruses was observed. These data suggest that, in PPV infections, symptom severity and competitiveness are independent aspects and that spatial separation may contribute to the displacement of a recombinant virus.

Original languageEnglish (US)
Pages (from-to)2846-2851
Number of pages6
JournalJournal of General Virology
Volume88
Issue number10
DOIs
StatePublished - Oct 1 2007

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Plum Pox Virus
Coinfection
Viruses
N-acetyltalosaminuronic acid

All Science Journal Classification (ASJC) codes

  • Virology

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A chimeric plum pox virus shows reduced spread and cannot compete with its parental wild-type viruses in a mixed infection. / Dietrich, Christof; Al Abdallah, Qusai; Lintl, Lara; Pietruszka, Agnes; Maiss, Edgar.

In: Journal of General Virology, Vol. 88, No. 10, 01.10.2007, p. 2846-2851.

Research output: Contribution to journalArticle

Dietrich, Christof ; Al Abdallah, Qusai ; Lintl, Lara ; Pietruszka, Agnes ; Maiss, Edgar. / A chimeric plum pox virus shows reduced spread and cannot compete with its parental wild-type viruses in a mixed infection. In: Journal of General Virology. 2007 ; Vol. 88, No. 10. pp. 2846-2851.
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