A chitosan/β-glycerophosphate thermo-sensitive gel for the delivery of ellagic acid for the treatment of brain cancer

Sungwoo Kim, Satoru Nishimoto, Joel D. Bumgardner, Warren O. Haggard, M. Waleed Gaber, Yunzhi Yang

Research output: Contribution to journalArticle

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Abstract

We report here the development of a chitosan/β-glycerophosphate(Ch/β-GP) thermo-sensitive gel to deliver ellagic acid (EA) for cancer treatment. The properties of the Ch/β-GP gels were characterized regarding chemical structure, surface morphology, and viscoelasticity. In vitro EA release rate from the EA loaded Ch/β-GP gel and chitosan degradation rate were investigated. The anti-tumor effect of the EA loaded Ch/β-GP gel on brain cancer cells (human U87 glioblastomas and rat C6 glioma cells) was evaluated by examining cell viability. Cell number and activity were monitored by the MTS assay. The Ch/β-GP solution formed a heat-induced gel at body temperature, and the gelation temperature and time were affected by the final pH of the Ch/β-GP solution. The lysozyme increased the EA release rate by 2.5 times higher than that in the absence of lysozyme. Dialyzed chitosan solution with final pH 6.3 greatly reduced the β-GP needed for gelation, thereby significantly improving the biocompatibility of gel (p < 0.001). The chitosan gels containing 1% (w/v) of ellagic acid significantly reduced viability of U87 cells and C6 cells compared with the chitosan gels at 3 days incubation (p < 0.01, and p < 0.001, respectively).

Original languageEnglish (US)
Pages (from-to)4157-4166
Number of pages10
JournalBiomaterials
Volume31
Issue number14
DOIs
StatePublished - May 1 2010

Fingerprint

Ellagic acid
Ellagic Acid
Glycerophosphates
Chitosan
Brain Neoplasms
Brain
Gels
Gelation
Muramidase
Cell Survival
Enzymes
Cells
Oncology
Viscoelasticity
Glioblastoma
Body Temperature
Biocompatibility
Glioma
Surface morphology
Rats

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

A chitosan/β-glycerophosphate thermo-sensitive gel for the delivery of ellagic acid for the treatment of brain cancer. / Kim, Sungwoo; Nishimoto, Satoru; Bumgardner, Joel D.; Haggard, Warren O.; Gaber, M. Waleed; Yang, Yunzhi.

In: Biomaterials, Vol. 31, No. 14, 01.05.2010, p. 4157-4166.

Research output: Contribution to journalArticle

Kim, Sungwoo ; Nishimoto, Satoru ; Bumgardner, Joel D. ; Haggard, Warren O. ; Gaber, M. Waleed ; Yang, Yunzhi. / A chitosan/β-glycerophosphate thermo-sensitive gel for the delivery of ellagic acid for the treatment of brain cancer. In: Biomaterials. 2010 ; Vol. 31, No. 14. pp. 4157-4166.
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