A comparison of intradermal and intravenous inoculation of bluetongue virus serotype 23 in sheep for clinico-pathology, and viral and immune responses

Channakeshava Sokke Umeshappa, Karam Pal Singh, Rudragouda Channappanavar, Kavita Sharma, Roopa Hebbandi Nanjundappa, Meeta Saxena, Rajendra Singh, Anil Kumar Sharma

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The pathogenesis of bluetongue (BT) could vary with route of inoculation. Using laboratory-passaged moderately virulent bluetongue virus serotype 23 (BTV-23), one of the most prevalent Indian serotype, we investigated the pathogenesis of BT in intradermally (ID) and intravenously (IV) inoculated native sheep. The ID inoculation resulted in relatively increased clinical signs and lesions in many organs as compared to IV inoculation. BTV-23 detection by real-time RT-PCR and isolation studies revealed that ID inoculation can be more efficient than IV ones in disseminating and spreading virus to systemic organs, including pre-scapular draining lymph node, spleen, lungs and pulmonary artery. Furthermore, the ID inoculation resulted in early onset and increased humoral response with significant increase (P<0.01) in antibody titre at various intervals. Taken together, these data suggest that ID inoculation can be more potent in reproducing many aspects of natural infection, including clinical disease, viral and immune responses, and may be useful route in setting up experimental infections for challenge or pathogenesis studies using laboratory passaged BTVs.

Original languageEnglish (US)
Pages (from-to)230-238
Number of pages9
JournalVeterinary Immunology and Immunopathology
Volume141
Issue number3-4
DOIs
StatePublished - Jun 15 2011

Fingerprint

Bluetongue virus
Bluetongue
Sheep
serotypes
bluetongue
pathogenesis
immune response
Pathology
sheep
pulmonary artery
Immune System Diseases
Virus Diseases
Infection
humoral immunity
lesions (animal)
infection
Pulmonary Artery
lymph nodes
Real-Time Polymerase Chain Reaction
spleen

All Science Journal Classification (ASJC) codes

  • Immunology
  • veterinary(all)

Cite this

A comparison of intradermal and intravenous inoculation of bluetongue virus serotype 23 in sheep for clinico-pathology, and viral and immune responses. / Umeshappa, Channakeshava Sokke; Singh, Karam Pal; Channappanavar, Rudragouda; Sharma, Kavita; Nanjundappa, Roopa Hebbandi; Saxena, Meeta; Singh, Rajendra; Sharma, Anil Kumar.

In: Veterinary Immunology and Immunopathology, Vol. 141, No. 3-4, 15.06.2011, p. 230-238.

Research output: Contribution to journalArticle

Umeshappa, Channakeshava Sokke ; Singh, Karam Pal ; Channappanavar, Rudragouda ; Sharma, Kavita ; Nanjundappa, Roopa Hebbandi ; Saxena, Meeta ; Singh, Rajendra ; Sharma, Anil Kumar. / A comparison of intradermal and intravenous inoculation of bluetongue virus serotype 23 in sheep for clinico-pathology, and viral and immune responses. In: Veterinary Immunology and Immunopathology. 2011 ; Vol. 141, No. 3-4. pp. 230-238.
@article{ff978e28e0aa4e7f9385d42028cd5f0b,
title = "A comparison of intradermal and intravenous inoculation of bluetongue virus serotype 23 in sheep for clinico-pathology, and viral and immune responses",
abstract = "The pathogenesis of bluetongue (BT) could vary with route of inoculation. Using laboratory-passaged moderately virulent bluetongue virus serotype 23 (BTV-23), one of the most prevalent Indian serotype, we investigated the pathogenesis of BT in intradermally (ID) and intravenously (IV) inoculated native sheep. The ID inoculation resulted in relatively increased clinical signs and lesions in many organs as compared to IV inoculation. BTV-23 detection by real-time RT-PCR and isolation studies revealed that ID inoculation can be more efficient than IV ones in disseminating and spreading virus to systemic organs, including pre-scapular draining lymph node, spleen, lungs and pulmonary artery. Furthermore, the ID inoculation resulted in early onset and increased humoral response with significant increase (P<0.01) in antibody titre at various intervals. Taken together, these data suggest that ID inoculation can be more potent in reproducing many aspects of natural infection, including clinical disease, viral and immune responses, and may be useful route in setting up experimental infections for challenge or pathogenesis studies using laboratory passaged BTVs.",
author = "Umeshappa, {Channakeshava Sokke} and Singh, {Karam Pal} and Rudragouda Channappanavar and Kavita Sharma and Nanjundappa, {Roopa Hebbandi} and Meeta Saxena and Rajendra Singh and Sharma, {Anil Kumar}",
year = "2011",
month = "6",
day = "15",
doi = "10.1016/j.vetimm.2011.03.005",
language = "English (US)",
volume = "141",
pages = "230--238",
journal = "Veterinary Immunology and Immunopathology",
issn = "0165-2427",
publisher = "Elsevier",
number = "3-4",

}

TY - JOUR

T1 - A comparison of intradermal and intravenous inoculation of bluetongue virus serotype 23 in sheep for clinico-pathology, and viral and immune responses

AU - Umeshappa, Channakeshava Sokke

AU - Singh, Karam Pal

AU - Channappanavar, Rudragouda

AU - Sharma, Kavita

AU - Nanjundappa, Roopa Hebbandi

AU - Saxena, Meeta

AU - Singh, Rajendra

AU - Sharma, Anil Kumar

PY - 2011/6/15

Y1 - 2011/6/15

N2 - The pathogenesis of bluetongue (BT) could vary with route of inoculation. Using laboratory-passaged moderately virulent bluetongue virus serotype 23 (BTV-23), one of the most prevalent Indian serotype, we investigated the pathogenesis of BT in intradermally (ID) and intravenously (IV) inoculated native sheep. The ID inoculation resulted in relatively increased clinical signs and lesions in many organs as compared to IV inoculation. BTV-23 detection by real-time RT-PCR and isolation studies revealed that ID inoculation can be more efficient than IV ones in disseminating and spreading virus to systemic organs, including pre-scapular draining lymph node, spleen, lungs and pulmonary artery. Furthermore, the ID inoculation resulted in early onset and increased humoral response with significant increase (P<0.01) in antibody titre at various intervals. Taken together, these data suggest that ID inoculation can be more potent in reproducing many aspects of natural infection, including clinical disease, viral and immune responses, and may be useful route in setting up experimental infections for challenge or pathogenesis studies using laboratory passaged BTVs.

AB - The pathogenesis of bluetongue (BT) could vary with route of inoculation. Using laboratory-passaged moderately virulent bluetongue virus serotype 23 (BTV-23), one of the most prevalent Indian serotype, we investigated the pathogenesis of BT in intradermally (ID) and intravenously (IV) inoculated native sheep. The ID inoculation resulted in relatively increased clinical signs and lesions in many organs as compared to IV inoculation. BTV-23 detection by real-time RT-PCR and isolation studies revealed that ID inoculation can be more efficient than IV ones in disseminating and spreading virus to systemic organs, including pre-scapular draining lymph node, spleen, lungs and pulmonary artery. Furthermore, the ID inoculation resulted in early onset and increased humoral response with significant increase (P<0.01) in antibody titre at various intervals. Taken together, these data suggest that ID inoculation can be more potent in reproducing many aspects of natural infection, including clinical disease, viral and immune responses, and may be useful route in setting up experimental infections for challenge or pathogenesis studies using laboratory passaged BTVs.

UR - http://www.scopus.com/inward/record.url?scp=79955581537&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955581537&partnerID=8YFLogxK

U2 - 10.1016/j.vetimm.2011.03.005

DO - 10.1016/j.vetimm.2011.03.005

M3 - Article

C2 - 21511346

AN - SCOPUS:79955581537

VL - 141

SP - 230

EP - 238

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

SN - 0165-2427

IS - 3-4

ER -