A deubiquitinating enzyme, UCH/CeUBP130, has an essential role in the formation of a functional microtubule-organizing centre (MTOC) during early cleavage in C. elegans

Jungsoo Lee, Chang Hoon Jee, Jin Il Lee, Myon Hee Lee, Moon Hee Lee, Hyeon Sook Koo, Chin Ha Chung, Joohong Ahnn

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background; Deubiquitinating enzymes generate monomeric ubiquitin in protein degradation pathways and are known to be important for the early development in many organisms. Results: RNA interference experiments targeted for a UBP homologue, UCH/CeUBP130, in C. elegans resulted in cell division defective embryos. Immunostaining localized UCH/CeUBP130 in the sperm and at the microtubule-organizing centre (MTOC) during early cleavage. Furthermore, the embryonic lethal phenotype was rescued by mating with wild-type males. Conclusions: Since it is known that the MTOC in the fertilized embryo is contributed by sperm asters in C. elegans, we suggest that UCH/CeUBP130 and ubiquitin protein degradation pathways may be involved in microtubule-based sperm aster formation. Therefore UCH/CeUBP130 is necessary for the formation of a functional MTOC in the fertilized embryo of C. elegans.

Original languageEnglish (US)
Pages (from-to)899-911
Number of pages13
JournalGenes to Cells
Volume6
Issue number10
DOIs
StatePublished - Nov 12 2001

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Microtubule-Organizing Center
Spermatozoa
Embryonic Structures
Ubiquitin
Proteolysis
RNA Interference
Microtubules
Cell Division
Phenotype
Deubiquitinating Enzymes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

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A deubiquitinating enzyme, UCH/CeUBP130, has an essential role in the formation of a functional microtubule-organizing centre (MTOC) during early cleavage in C. elegans. / Lee, Jungsoo; Jee, Chang Hoon; Lee, Jin Il; Lee, Myon Hee; Lee, Moon Hee; Koo, Hyeon Sook; Chung, Chin Ha; Ahnn, Joohong.

In: Genes to Cells, Vol. 6, No. 10, 12.11.2001, p. 899-911.

Research output: Contribution to journalArticle

Lee, Jungsoo ; Jee, Chang Hoon ; Lee, Jin Il ; Lee, Myon Hee ; Lee, Moon Hee ; Koo, Hyeon Sook ; Chung, Chin Ha ; Ahnn, Joohong. / A deubiquitinating enzyme, UCH/CeUBP130, has an essential role in the formation of a functional microtubule-organizing centre (MTOC) during early cleavage in C. elegans. In: Genes to Cells. 2001 ; Vol. 6, No. 10. pp. 899-911.
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abstract = "Background; Deubiquitinating enzymes generate monomeric ubiquitin in protein degradation pathways and are known to be important for the early development in many organisms. Results: RNA interference experiments targeted for a UBP homologue, UCH/CeUBP130, in C. elegans resulted in cell division defective embryos. Immunostaining localized UCH/CeUBP130 in the sperm and at the microtubule-organizing centre (MTOC) during early cleavage. Furthermore, the embryonic lethal phenotype was rescued by mating with wild-type males. Conclusions: Since it is known that the MTOC in the fertilized embryo is contributed by sperm asters in C. elegans, we suggest that UCH/CeUBP130 and ubiquitin protein degradation pathways may be involved in microtubule-based sperm aster formation. Therefore UCH/CeUBP130 is necessary for the formation of a functional MTOC in the fertilized embryo of C. elegans.",
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AU - Lee, Jungsoo

AU - Jee, Chang Hoon

AU - Lee, Jin Il

AU - Lee, Myon Hee

AU - Lee, Moon Hee

AU - Koo, Hyeon Sook

AU - Chung, Chin Ha

AU - Ahnn, Joohong

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