A factor from damaged rat kidney stimulates collagen biosynthesis by mesangial cells

Kunio Ohyama, Jerome M. Seyer, Rajendra Raghow, Andrew Kang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Rats were administered CCl4, a well-defined nephrotoxin, for 20 weeks to produce glomerular sclerosis. Tubular degeneration and necrosis with interstitial fibrosis was clearly evident by histological examination. Kidneys were homogenized in phosphate-buffered saline and a collagen synthesis-stimulating factor was isolated by Sephadex G-50 gel filtration. The 5 kDa component stimulated both type I and type IV procollagen synthesis by mesangial cells and type I procollagen synthesis by rat skin fibroblasts. In each cell type, 2-6-fold increases in procollagen protein production or cell proliferation was noted. The steady-state levels of mRNA encoding for procollagen α1(I) and procollagen α1(IV) chains in mesangial cells were determined by hybridization to their corresponding cDNA clones. The type I procollagen mRNA was elevated 1.4-fold compared to a 1.6-fold increase in mRNA encoding for type IV procollagen. The similar properties and chemical characteristics of this fibrogenic factor with a factor from fibrotic liver suggests they are the same and that a common endogenous collagen synthesis stimulator may be present in fibrosing organs, thus providing a driving force for collagen over-production.

Original languageEnglish (US)
Pages (from-to)173-178
Number of pages6
JournalBBA - Molecular Cell Research
Volume1053
Issue number2-3
DOIs
StatePublished - Jul 12 1990

Fingerprint

Procollagen
Mesangial Cells
Collagen Type I
Collagen
Collagen Type IV
Kidney
Messenger RNA
Sclerosis
Gel Chromatography
Fibrosis
Necrosis
Complementary DNA
Clone Cells
Fibroblasts
Phosphates
Cell Proliferation
Skin
Liver
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

A factor from damaged rat kidney stimulates collagen biosynthesis by mesangial cells. / Ohyama, Kunio; Seyer, Jerome M.; Raghow, Rajendra; Kang, Andrew.

In: BBA - Molecular Cell Research, Vol. 1053, No. 2-3, 12.07.1990, p. 173-178.

Research output: Contribution to journalArticle

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