A ferret model of synergism between influenza virus and Streptococcus pneumoniae

Ville T. Peltola, Jerold E. Rehg, Jonathan Mccullers

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Mouse-adapted laboratory strains of influenza virus have to be used in mouse models of synergism between influenza virus and Streptococcus pneumoniae. Since ferrets support human influenza viruses without adaptation, we sought to characterize a ferret model of synergism between influenza and pneumococcus. Methods: Pairs of ferrets were infected with either influenza virus A/Sydney/5/97 or PBS then challenged 5 days later with either pneumococcus strain D39 or PBS. Clinical and laboratory parameters were monitored during a 48-h follow-up period after the challenge. Results: Ferrets receiving influenza then pneumococcus exhibited more severe clinical signs of illness and larger increases in WBC and segmental cell counts than did ferrets receiving influenza virus or pneumococcus alone. Pneumococcal nasal wash titers were higher in ferrets infected with influenza virus followed by pneumococcus than in those infected with pneumococcus alone. Histopathologic abnormalities in lungs were mild, but differences between sequentially infected and singly infected ferrets were observed. Conclusion: A sequential infection with influenza virus and pneumococcus causes a synergistic increase in morbidity in ferrets. This ferret model will be useful for study of the interactions between non-adapted human influenza viruses and S. pneumoniae.

Original languageEnglish (US)
Pages (from-to)486-490
Number of pages5
JournalInternational Congress Series
Volume1263
Issue numberC
DOIs
StatePublished - Jun 1 2004
Externally publishedYes

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Ferrets
Streptococcus pneumoniae
Orthomyxoviridae
Human Influenza
Influenza A virus
Nose
Cell Count
Morbidity
Lung

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

A ferret model of synergism between influenza virus and Streptococcus pneumoniae. / Peltola, Ville T.; Rehg, Jerold E.; Mccullers, Jonathan.

In: International Congress Series, Vol. 1263, No. C, 01.06.2004, p. 486-490.

Research output: Contribution to journalArticle

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