A fungus-specific Ras homolog contributes to the hyphal growth and virulence of Aspergillus fumigatus

Jarrod Fortwendel, Wei Zhao, Ruchi Bhabhra, Steven Park, David S. Perlin, David S. Askew, Judith C. Rhodes

Research output: Contribution to journalArticle

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Abstract

The Ras family of GTPase proteins has been shown to control morphogenesis in many organisms, including several species of pathogenic fungi. In a previous study, we identified a gene encoding a fungus-specific Ras subfamily homolog, rasB, in Aspergillus fumigatus. Here we report that deletion of A. fumigatus rasB caused decreased germination and growth rates on solid media but had no effect on total biomass accumulation after 24 h of growth in liquid culture. The ΔrasB mutant had an irregular hyphal morphology characterized by increased branching. Expression of rasBΔ113-135, a mutant transgene lacking the conserved rasB internal amino acid insertion, did not complement the deletion phenotype of delayed growth and germination rates and abnormal hyphal morphology. Virulence of the rasB deletion strain was diminished; mice infected with this strain exhibited ∼65% survival compared to ∼10% with wild-type and reconstituted strains. These data support the hypothesis that rasB homologs, which are highly conserved among fungi that undergo hyphal growth, control signaling modules important to the directional growth of fungal hyphae.

Original languageEnglish (US)
Pages (from-to)1982-1989
Number of pages8
JournalEukaryotic Cell
Volume4
Issue number12
DOIs
StatePublished - Dec 1 2005

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Aspergillus fumigatus
Virulence
Fungi
Growth
Germination
ras Proteins
Hyphae
Transgenes
Morphogenesis
Biomass
Phenotype
Amino Acids
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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A fungus-specific Ras homolog contributes to the hyphal growth and virulence of Aspergillus fumigatus. / Fortwendel, Jarrod; Zhao, Wei; Bhabhra, Ruchi; Park, Steven; Perlin, David S.; Askew, David S.; Rhodes, Judith C.

In: Eukaryotic Cell, Vol. 4, No. 12, 01.12.2005, p. 1982-1989.

Research output: Contribution to journalArticle

Fortwendel, Jarrod ; Zhao, Wei ; Bhabhra, Ruchi ; Park, Steven ; Perlin, David S. ; Askew, David S. ; Rhodes, Judith C. / A fungus-specific Ras homolog contributes to the hyphal growth and virulence of Aspergillus fumigatus. In: Eukaryotic Cell. 2005 ; Vol. 4, No. 12. pp. 1982-1989.
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