A low-cost solution to measure mouse licking in an electrophysiological setup with a standard analog-to-digital converter

Abdallah Hayar, Jeri L. Bryant, John Boughter, Detlef Heck

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Licking behavior in rodents is widely used to determine fluid consumption in various behavioral contexts and is a typical example of rhythmic movement controlled by internal pattern-generating mechanisms. The measurement of licking behavior by commercially available instruments is based on either tongue protrusion interrupting a light beam or on an electrical signal generated by the tongue touching a metal spout. We report here that licking behavior can be measured with high temporal precision by simply connecting a metal sipper tube to the input of a standard analog/digital (A/D) converter and connecting the animal to ground (via a metal cage floor). The signal produced by a single lick consists of a 100-800 mV dc voltage step, which reflects the metal-to-water junction potential and persists for the duration of the tongue-spout contact. This method does not produce any significant electrical artifacts and can be combined with electrophysiological measurements of single unit activity from neurons involved in the control of the licking behavior.

Original languageEnglish (US)
Pages (from-to)203-207
Number of pages5
JournalJournal of Neuroscience Methods
Volume153
Issue number2
DOIs
StatePublished - Jun 15 2006

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Metals
Tongue
Costs and Cost Analysis
Behavior Control
Artifacts
Rodentia
Neurons
Light
Water

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

A low-cost solution to measure mouse licking in an electrophysiological setup with a standard analog-to-digital converter. / Hayar, Abdallah; Bryant, Jeri L.; Boughter, John; Heck, Detlef.

In: Journal of Neuroscience Methods, Vol. 153, No. 2, 15.06.2006, p. 203-207.

Research output: Contribution to journalArticle

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