A luxCDABE-based bioluminescent bioreporter for the detection of phenol

D. Abd-El-Haleem, S. Ripp, J. Scott, Gary S. Sayler

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

A bioluminescent reporter strain, Acinetobacter sp. DF4-8, was constructed for the detection of phenol by inserting a mopR-like promoter upstream of the Vibrio fischeri bioluminescent luxCDABE gene cassette in a modified mini-Tn5 construct. When introduced into the chromosome of Acinetobacter sp. DF4, the bioreporter produced a sensitive bioluminescent response to phenol at concentrations ranging from 2.5 to 100 ppm. This response was linear (R 2 =0.986) in the range from 20 to 90 ppm. A significant bioluminescent response was also recorded when strain DF4-8 was incubated with slurries from aged, phenol-contaminated soil.

Original languageEnglish (US)
Pages (from-to)233-237
Number of pages5
JournalJournal of Industrial Microbiology and Biotechnology
Volume29
Issue number5
DOIs
StatePublished - Nov 1 2002
Externally publishedYes

Fingerprint

Phenol
Phenols
Acinetobacter
Aliivibrio fischeri
Slurries
Chromosomes
Soil
Genes
Soils

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

A luxCDABE-based bioluminescent bioreporter for the detection of phenol. / Abd-El-Haleem, D.; Ripp, S.; Scott, J.; Sayler, Gary S.

In: Journal of Industrial Microbiology and Biotechnology, Vol. 29, No. 5, 01.11.2002, p. 233-237.

Research output: Contribution to journalArticle

Abd-El-Haleem, D. ; Ripp, S. ; Scott, J. ; Sayler, Gary S. / A luxCDABE-based bioluminescent bioreporter for the detection of phenol. In: Journal of Industrial Microbiology and Biotechnology. 2002 ; Vol. 29, No. 5. pp. 233-237.
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