A major epitope of synthetic rabbit encephalitogen S24 disclosed through the use of a tritiated peptide probe

S. J. Tyrey, Nicholas Potter, E. D. Day, G. A. Hashim

Research output: Contribution to journalArticle

Abstract

The antibody recognition of synthetic rabbit encephalitogen S24 (TTHYGSLPQKG), which had been inactivated by extensive iodination, was fully restored by tritium‐iodine exchange during boron hydride reduction. A highly active form of 3H‐S24 was obtained by reverse‐phase high‐performance liquid chromatography (HPLC) purification of the crude product and could be further separted into three continuous subfractions, the latter two of which were fully active from an immunochemical point of view. A major epitope was diclosed through use of the 3H‐S24 probe, which required the intact S24 peptide for its expression. Confirmation of two linear epitopes previously encountered, GSLPQK and THYGSL, was also obtained, the latter through the use of the third HPLC subfraction of 3H‐S24.

Original languageEnglish (US)
Pages (from-to)493-496
Number of pages4
JournalJournal of Neuroscience Research
Volume18
Issue number3
DOIs
StatePublished - Jan 1 1987
Externally publishedYes

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Liquid Chromatography
Epitopes
Boranes
Rabbits
Peptides
Halogenation
Antibodies

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience

Cite this

A major epitope of synthetic rabbit encephalitogen S24 disclosed through the use of a tritiated peptide probe. / Tyrey, S. J.; Potter, Nicholas; Day, E. D.; Hashim, G. A.

In: Journal of Neuroscience Research, Vol. 18, No. 3, 01.01.1987, p. 493-496.

Research output: Contribution to journalArticle

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