A Monoclonal Antibody to a Carbohydrate Epitope Expressed on Glycolipid and on α3β1 Integrin on Human Esophageal Carcinoma

Roudabeh J. Jamasbi, Gary D. Stoner, Linda J. Foote, Trish K. Lankford, Sandra Davern, Stephen Kennel

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A mouse monoclonal antibody (MAb-9) produced by immunization with a human esophageal carcinoma cell line, TE-2 (derived from undifferentiated squamous cell carcinoma) reacted specifically with about 30% of esophageal carcinoma cell lines and tissue sections from clinical samples. MAb-9 showed minimal reactivity with normal esophageal tissue. 125I, fluorescent or gold particle labeled MAb-9 bound to TE-2 cell surfaces. 125I- radiolabeled MAb-9 was used to detect reactive material from cell extracts in Western blot. Treatment of TE-2 membrane proteins with neuraminidase, N-glycanase or O-glycanase reduced antigen detection. Treatment of cells with periodic acid destroyed antibody binding in ELISA. Lipid extracts from cell membranes, containing glycolipids, also reacted with MAb-9. MAb-9 was used to purify target antigen from detergent solubilized membrane proteins and the prominent bands from subsequent gel electrophoresis were trypsin digested and analyzed by mass spectrometry. Peptides from α3 and β 1 integrin chains were identified. These data indicate that α3β1 integrin is prominently expressed on certain esophageal carcinomas and that a specific carbohydrate unit is selectively displayed on the α3 integrin subunit as well as on glycolipid on the cell surface. The α3β1 integrin expressed on A-431 carcinoma cells does not display this carbohydrate epitope and is not detected by MAb-9. Thus, expression of the carbohydrate epitope is the basis for the tumor selective reaction of MAb-9 with a subset of esophageal carcinomas.

Original languageEnglish (US)
Pages (from-to)367-376
Number of pages10
JournalHybridoma and Hybridomics
Volume22
Issue number6
DOIs
StatePublished - Jan 1 2003
Externally publishedYes

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Glycolipids
Integrins
Epitopes
Monoclonal Antibodies
Carbohydrates
Carcinoma
Membrane Proteins
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Antigens
Cell Line
Periodic Acid
Neuraminidase
Membrane Lipids
Cell Extracts
Gold
Detergents
Trypsin
Electrophoresis
Squamous Cell Carcinoma
Immunization

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

Cite this

A Monoclonal Antibody to a Carbohydrate Epitope Expressed on Glycolipid and on α3β1 Integrin on Human Esophageal Carcinoma. / Jamasbi, Roudabeh J.; Stoner, Gary D.; Foote, Linda J.; Lankford, Trish K.; Davern, Sandra; Kennel, Stephen.

In: Hybridoma and Hybridomics, Vol. 22, No. 6, 01.01.2003, p. 367-376.

Research output: Contribution to journalArticle

Jamasbi, Roudabeh J. ; Stoner, Gary D. ; Foote, Linda J. ; Lankford, Trish K. ; Davern, Sandra ; Kennel, Stephen. / A Monoclonal Antibody to a Carbohydrate Epitope Expressed on Glycolipid and on α3β1 Integrin on Human Esophageal Carcinoma. In: Hybridoma and Hybridomics. 2003 ; Vol. 22, No. 6. pp. 367-376.
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