A mouse model of dual infection with influenza virus and Streptococcus pneumoniae

Jonathan Mccullers, Robert G. Webster

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: A lethal synergism exists between influenza virus and Streptococcus pneumoniae accounting for excess mortality during influenza epidemics. A small animal model of dual infection with these organisms would be useful for study of pathogenic mechanisms underlying this interaction. Methods: Groups of mice were infected with either mouse adapted influenza virus A/Puerto Rico/8/34 (H1N1), S. pneumoniae strain D39, both simultaneously, or pneumococcus following influenza virus. Weight loss, as a measure of morbidity, and mortality were followed. Blood cultures were collected 24 h after infection. Results: Mice infected simultaneously with both influenza virus and pneumococcus exhibited gradual weight loss and mortality commensurate with expectations for an additive process. In contrast, mice infected with pneumococcus 7 days following infection with influenza virus uniformly died in less than 24 h and were highly bacteremic. Discussion: A mouse model of sequential infection with influenza virus and S. pneumoniae has been developed. Mice infected with pneumococcus seven days after infection with influenza virus exhibit a synergistic lethality caused by overwhelming sepsis. This model will be useful for study of the mechanisms involved in pathogenic interactions between influenza virus and pneumococcus.

Original languageEnglish (US)
Pages (from-to)601-607
Number of pages7
JournalInternational Congress Series
Volume1219
Issue numberC
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

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Streptococcus pneumoniae
Orthomyxoviridae
Infection
Mortality
Weight Loss
Puerto Rico
Influenza A virus
Human Influenza
Sepsis
Animal Models
Morbidity

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

A mouse model of dual infection with influenza virus and Streptococcus pneumoniae. / Mccullers, Jonathan; Webster, Robert G.

In: International Congress Series, Vol. 1219, No. C, 01.01.2001, p. 601-607.

Research output: Contribution to journalArticle

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