A murine hypertrophic cardiomyopathy model

The DBA/2J strain

Wenyuan Zhao, Tieqiang Zhao, Yuanjian Chen, Fengbo Zhao, Qingqing Gu, Robert Williams, Syamal Bhattacharya, Lu Lu, Yao Sun

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Familial hypertrophic cardiomyopathy (HCM) is attributed to mutations in genes that encode for the sarcomere proteins, especially Mybpc3 and Myh7. Genotype-phenotype correlation studies show significant variability in HCM phenotypes among affected individuals with identical causal mutations. Morphological changes and clinical expression of HCM are the result of interactions with modifier genes. With the exceptions of angiotensin converting enzyme, these modifiers have not been identified. Although mouse models have been used to investigate the genetics of many complex diseases, natural murine models for HCM are still lacking. In this study we show that the DBA/2J (D2) strain of mouse has sequence variants in Mybpc3 and Myh7, relative to widely used C57BL/6J (B6) reference strain and the key features of human HCM. Four-month-old of male D2 mice exhibit hallmarks of HCM including increased heart weight and cardiomyocyte size relative to B6 mice, as well as elevated markers for cardiac hypertrophy including β-myosin heavy chain (MHC), atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and skeletal muscle alpha actin (α1-actin). Furthermore, cardiac interstitial fibrosis, another feature of HCM, is also evident in the D2 strain, and is accompanied by up-regulation of type I collagen and α-smooth muscle actin (SMA)-markers of fibrosis. Of great interest, blood pressure and cardiac function are within the normal range in the D2 strain, demonstrating that cardiac hypertrophy and fibrosis are not secondary to hypertension, myocardial infarction, or heart failure. Because D2 and B6 strains have been used to generate a large family of recombinant inbred strains, the BXD cohort, the D2 model can be effectively exploited for in-depth genetic analysis of HCM susceptibility and modifier screens.

Original languageEnglish (US)
Article numbere0133132
JournalPLoS ONE
Volume10
Issue number8
DOIs
StatePublished - Aug 4 2015

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Hypertrophic Cardiomyopathy
cardiomyopathy
mice
modifiers (genes)
Actins
fibrosis
Fibrosis
actin
Cardiomegaly
Muscle
hypertrophy
Heart Failure
Familial Hypertrophic Cardiomyopathy
Modifier Genes
Myosin Heavy Chains
Brain Natriuretic Peptide
animal models
Blood pressure
Atrial Natriuretic Factor
Peptidyl-Dipeptidase A

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

A murine hypertrophic cardiomyopathy model : The DBA/2J strain. / Zhao, Wenyuan; Zhao, Tieqiang; Chen, Yuanjian; Zhao, Fengbo; Gu, Qingqing; Williams, Robert; Bhattacharya, Syamal; Lu, Lu; Sun, Yao.

In: PLoS ONE, Vol. 10, No. 8, e0133132, 04.08.2015.

Research output: Contribution to journalArticle

Zhao, Wenyuan ; Zhao, Tieqiang ; Chen, Yuanjian ; Zhao, Fengbo ; Gu, Qingqing ; Williams, Robert ; Bhattacharya, Syamal ; Lu, Lu ; Sun, Yao. / A murine hypertrophic cardiomyopathy model : The DBA/2J strain. In: PLoS ONE. 2015 ; Vol. 10, No. 8.
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