A murine model of Candida glabrata vaginitis shows no evidence of an inflammatory immunopathogenic response

Evelyn E. Nash, Brian Peters, Elizabeth A. Lilly, Mairi C. Noverr, Paul L. Fidel

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Candida glabrata is the second most common organism isolated from women with vulvovaginal candidiasis (VVC), particularly in women with uncontrolled diabetes mellitus. However, mechanisms involved in the pathogenesis of C. glabrata-associated VVC are unknown and have not been studied at any depth in animal models. The objective of this study was to evaluate host responses to infection following efforts to optimize a murine model of C. glabrata VVC. For this, various designs were evaluated for consistent experimental vaginal colonization (i.e., type 1 and type 2 diabetic mice, exogenous estrogen, varying inocula, and co-infection with C. albicans). Upon model optimization, vaginal fungal burden and polymorphonuclear neutrophil (PMN) recruitment were assessed longitudinally over 21 days post-inoculation, together with vaginal concentrations of IL-1β, S100A8 alarmin, lactate dehydrogenase (LDH), and in vivo biofilm formation. Consistent and sustained vaginal colonization with C. glabrata was achieved in estrogenized streptozotocin-induced type 1 diabetic mice. Vaginal PMN infiltration was consistently low, with IL-1β, S100A8, and LDH concentrations similar to uninoculated mice. Biofilm formation was not detected in vivo, and co-infection with C. albicans did not induce synergistic immunopathogenic effects. This data suggests that experimental vaginal colonization of C. glabrata is not associated with an inflammatory immunopathogenic response or biofilm formation.

Original languageEnglish (US)
Article numbere0147969
JournalPLoS One
Volume11
Issue number1
DOIs
StatePublished - Jan 1 2016

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vaginitis
Candida glabrata
Vaginitis
Candida
Biofilms
Vulvovaginal Candidiasis
inflammation
animal models
Interleukin-1
L-Lactate Dehydrogenase
biofilm
Neutrophil Infiltration
interleukin-1
lactate dehydrogenase
Coinfection
mixed infection
neutrophils
Streptozocin
Medical problems
mice

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

A murine model of Candida glabrata vaginitis shows no evidence of an inflammatory immunopathogenic response. / Nash, Evelyn E.; Peters, Brian; Lilly, Elizabeth A.; Noverr, Mairi C.; Fidel, Paul L.

In: PLoS One, Vol. 11, No. 1, e0147969, 01.01.2016.

Research output: Contribution to journalArticle

Nash, Evelyn E. ; Peters, Brian ; Lilly, Elizabeth A. ; Noverr, Mairi C. ; Fidel, Paul L. / A murine model of Candida glabrata vaginitis shows no evidence of an inflammatory immunopathogenic response. In: PLoS One. 2016 ; Vol. 11, No. 1.
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