A neuroanatomical gradient in the pontine tegmentum for the cholinoceptive induction of desynchronized sleep signs

Helen Baghdoyan, Margarita L. Rodrigo-Angulo, Robert W. McCarley, J. Allan Hobson

Research output: Contribution to journalArticle

160 Citations (Scopus)

Abstract

Microinjection of cholinergic agonists into the tegmentum was used to evoke a state which was polygraphically and behaviorally similar to desynchronized (D) sleep. This study was designed to test the hypothesis that the production of this pharmacologically induced D sleep-like state (D-ACh) was dependent upon the anatomical locus of drug administration within the pottine tegmentum. Four dependent variables of D sleep were measured: D latency, D percentage, D duration and D frequency. Multiple regression analysis and analysis of variance were performed to evaluate the relationship between the three-dimensional coordinates of the injection site (posterior, vertical and lateral) and these 4 dependent measures. The intrapontine site of drug administration accounted for a statistically significant amount of the variance in D latency, D percentage and D duration. There was no significant relationship between the anatomical site of saline injection and the dependent measures of D sleep. A significance increase in D frequency following microinjection of cholinergic agonists was found to be independent of injection site. Pontine injection sites which yielded the shortest D latencies were found to be the same sites from which the highest D percentages were evoked. Rostrodorsal pontine tegmental injection sites were most effective in producing the highest percentages of D-ACh with the shortest latencies to onset. Injections made more caudally and ventrally within the pontine tegmentum produced lower percentages of D-ACh with longer latencies to onset. These data suggest the existence of an anatomical gradient within the pontine tegmentum for the cholinoceptive evocation of a D sleep-like state, and further support the concept that D sleep is generated, in part, by pontine cholinergic mechanisms.

Original languageEnglish (US)
Pages (from-to)245-261
Number of pages17
JournalBrain Research
Volume414
Issue number2
DOIs
StatePublished - Jun 30 1987
Externally publishedYes

Fingerprint

Sleep
Injections
Cholinergic Agonists
Microinjections
Pharmaceutical Preparations
Cholinergic Agents
Pontine Tegmentum
Analysis of Variance
Regression Analysis

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

A neuroanatomical gradient in the pontine tegmentum for the cholinoceptive induction of desynchronized sleep signs. / Baghdoyan, Helen; Rodrigo-Angulo, Margarita L.; McCarley, Robert W.; Allan Hobson, J.

In: Brain Research, Vol. 414, No. 2, 30.06.1987, p. 245-261.

Research output: Contribution to journalArticle

Baghdoyan, Helen ; Rodrigo-Angulo, Margarita L. ; McCarley, Robert W. ; Allan Hobson, J. / A neuroanatomical gradient in the pontine tegmentum for the cholinoceptive induction of desynchronized sleep signs. In: Brain Research. 1987 ; Vol. 414, No. 2. pp. 245-261.
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