A neurogenomics approach to gene expression analysis in the developing brain

Patricia Jensen, Susan Magdaleno, Karen M. Lehman, Dennis S. Rice, Edward R. LaVallie, Lisa Collins-Racie, James Mccoy, Tom Curran

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Secreted and transmembrane proteins provide critical functions in the signaling networks essential for neurogenesis. We used a genetic signal sequence gene trap approach to isolate 189 genes expressed during development in e16.5 whole head, e16.5 hippocampus and e14.5 cerebellum. Gene ontology programs were used to classify the genes into respective biological processes. Four major classes of biological processes known to be important during development were identified: cell communication, cell physiology processes, metabolism and morphogenesis. We used in situ hybridization to determine the temporal and spatial patterns of gene expression in the developing brain using this set of probes. The results demonstrate that gene expression patterns can highlight potential gene functions in specific brain regions. We propose that combining bioinformatics with the gene expression pattern is an effective strategy to identify genes that may play critical roles during brain development.

Original languageEnglish (US)
Pages (from-to)116-127
Number of pages12
JournalMolecular Brain Research
Volume132
Issue number2
DOIs
StatePublished - Dec 20 2004
Externally publishedYes

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Gene Expression
Brain
Biological Phenomena
Genes
Cell Physiological Phenomena
Gene Ontology
Neurogenesis
Protein Sorting Signals
Computational Biology
Morphogenesis
Cell Communication
Cerebellum
In Situ Hybridization
Hippocampus
Head
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Jensen, P., Magdaleno, S., Lehman, K. M., Rice, D. S., LaVallie, E. R., Collins-Racie, L., ... Curran, T. (2004). A neurogenomics approach to gene expression analysis in the developing brain. Molecular Brain Research, 132(2), 116-127. https://doi.org/10.1016/j.molbrainres.2004.10.002

A neurogenomics approach to gene expression analysis in the developing brain. / Jensen, Patricia; Magdaleno, Susan; Lehman, Karen M.; Rice, Dennis S.; LaVallie, Edward R.; Collins-Racie, Lisa; Mccoy, James; Curran, Tom.

In: Molecular Brain Research, Vol. 132, No. 2, 20.12.2004, p. 116-127.

Research output: Contribution to journalArticle

Jensen, P, Magdaleno, S, Lehman, KM, Rice, DS, LaVallie, ER, Collins-Racie, L, Mccoy, J & Curran, T 2004, 'A neurogenomics approach to gene expression analysis in the developing brain', Molecular Brain Research, vol. 132, no. 2, pp. 116-127. https://doi.org/10.1016/j.molbrainres.2004.10.002
Jensen P, Magdaleno S, Lehman KM, Rice DS, LaVallie ER, Collins-Racie L et al. A neurogenomics approach to gene expression analysis in the developing brain. Molecular Brain Research. 2004 Dec 20;132(2):116-127. https://doi.org/10.1016/j.molbrainres.2004.10.002
Jensen, Patricia ; Magdaleno, Susan ; Lehman, Karen M. ; Rice, Dennis S. ; LaVallie, Edward R. ; Collins-Racie, Lisa ; Mccoy, James ; Curran, Tom. / A neurogenomics approach to gene expression analysis in the developing brain. In: Molecular Brain Research. 2004 ; Vol. 132, No. 2. pp. 116-127.
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