A new monoclonal antibody to study mouse macrophage antigen during BHT-induced lung injury and repair

Stephen Kennel, Trish Lankford, Pamela Galloway, H. P. Witschi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A rat monoclonal antibody 133-13A to a mouse lung carcinoma cell line was found to react with macrophages in mouse lung [1]. This monoclonal antibody is different from previously described antibodies to macrophages. Immunogold electron-microscopy and immunoperoxidase light microscopy have been used to show that MoAb 133-13A binds specifically to macrophages in normal and in BHT treated mouse lungs. This MoAb recognizes a protein of approximately 100 kDa (P100) on cultured lung carcinoma cells and a 87 kDa protein on macrophages from lung or the peritoneal cavity which is different from other marcophage using immunoaffinity chromatography. The purified protein was radioiodinated and MoAb 133-13A was used to develop a competition radioimmunoassay to quantitate P100. Spleen, intestine, lung, skin and uterus all have high levels of P100. P100 on peritoneal macrophages has been determined to be about 94 000 molecules/cell. Analyses of lung lavage and whole lung homogenates from mice treated with BHT, BHT plus 70% O2, and 70% O2 alone show that treated animals have elevated P100 content compared to corn oil treated mice.

Original languageEnglish (US)
Pages (from-to)103-116
Number of pages14
JournalToxicology
Volume55
Issue number1-2
DOIs
StatePublished - Jan 1 1989

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Butylated Hydroxytoluene
Macrophages
Lung Injury
Repair
Monoclonal Antibodies
Antigens
Lung
Cells
Proteins
Corn Oil
Chromatography
Carcinoma
Electron microscopy
Optical microscopy
Rats
Skin
Animals
Peritoneal Cavity
Peritoneal Macrophages
Bronchoalveolar Lavage

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

A new monoclonal antibody to study mouse macrophage antigen during BHT-induced lung injury and repair. / Kennel, Stephen; Lankford, Trish; Galloway, Pamela; Witschi, H. P.

In: Toxicology, Vol. 55, No. 1-2, 01.01.1989, p. 103-116.

Research output: Contribution to journalArticle

Kennel, Stephen ; Lankford, Trish ; Galloway, Pamela ; Witschi, H. P. / A new monoclonal antibody to study mouse macrophage antigen during BHT-induced lung injury and repair. In: Toxicology. 1989 ; Vol. 55, No. 1-2. pp. 103-116.
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