A new protocol for quantifying CD34+ Cells in peripheral blood of patients with cardiovascular disease

Akie Kikuchi-Taura, Toshihiro Soma, Tomohiro Matsuyama, David Stern, Akihiko Taguchi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Increasing evidence points to a role for circulating CD34-positive (CD34+) cells in vascular maintenance and neovascularization. Although there are established methods for evaluating absolute numbers of CD34+ cells in bone marrow or mobilized peripheral blood, there is no convenient and highly reproducible method for quantifying low numbers of CD34+ cells in blood samples, such as those from the peripheral blood of patients with cardiovascular disease. With current commonly used methods, the mean percentage of CD34+ cells in leukocyte fractions from such patients was only 0.02%, and the cumulative intra-assay coefficient of variation was ∼30%. With use of the protocol described herein, actual counts of CD34+ cells increased ∼5-fold and cumulative intra-assay coefficients of variation were reduced to ∼7%. The new method is useful to precisely measure low numbers of CD34+ cells in samples, and it has potential as a screening tool to evaluate cardiovascular risk in large patient populations.

Original languageEnglish (US)
Pages (from-to)427-429
Number of pages3
JournalTexas Heart Institute Journal
Volume33
Issue number4
StatePublished - 2006
Externally publishedYes

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Cardiovascular Diseases
Cell Count
Bone Marrow Cells
Blood Vessels
Blood Cells
Leukocytes
Maintenance
Population

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

A new protocol for quantifying CD34+ Cells in peripheral blood of patients with cardiovascular disease. / Kikuchi-Taura, Akie; Soma, Toshihiro; Matsuyama, Tomohiro; Stern, David; Taguchi, Akihiko.

In: Texas Heart Institute Journal, Vol. 33, No. 4, 2006, p. 427-429.

Research output: Contribution to journalArticle

Kikuchi-Taura, Akie ; Soma, Toshihiro ; Matsuyama, Tomohiro ; Stern, David ; Taguchi, Akihiko. / A new protocol for quantifying CD34+ Cells in peripheral blood of patients with cardiovascular disease. In: Texas Heart Institute Journal. 2006 ; Vol. 33, No. 4. pp. 427-429.
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