A novel knot method for individually measurable aortic constriction in rats

Jiaming Liu, Pengfei Han, Ying Xiao, Jiani Liu, Yujian Kang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A novel knot method in rats is reported that addresses several drawbacks in the current model of aortic constriction-induced heart hypertrophy. Using a rat model, we developed a two-step procedure that includes 1) measurement of individual aorta circumference using a surgical thread; and 2) constriction of the aorta using a thread with the desired length predefined by a knot at each end for a measurable reduction of the aortic circumference as referenced to the measurement in step 1. This knot approach produces a manageable gradient of aortic constriction in each rat, reaching a consistency among experimental animals that cannot be achieved by the traditional needle method. Notably, the animal model produced by our knot method showed cardiac hypertrophy and dysfunction with the severity proportional to the percentage reduction of the aorta circumference (50% vs. 60%). Additionally, our new procedure produced a lower mortality rate compared with the traditional needle method. Therefore, we recommend this knot method as an alternative procedure for aortic constriction with desired gradient in rats and larger-animal models.

Original languageEnglish (US)
Pages (from-to)H987-H995
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume307
Issue number7
DOIs
StatePublished - Oct 1 2014
Externally publishedYes

Fingerprint

Constriction
Aorta
Cardiomegaly
Needles
Animal Models
Mortality

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

A novel knot method for individually measurable aortic constriction in rats. / Liu, Jiaming; Han, Pengfei; Xiao, Ying; Liu, Jiani; Kang, Yujian.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 307, No. 7, 01.10.2014, p. H987-H995.

Research output: Contribution to journalArticle

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