A novel large conductance, nonselective cation channel in pheochromocytoma (PC12) cells

Alejandro Dopico, S. N. Treistman

Research output: Contribution to journalArticle

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Abstract

A new type of nonselective cation channel was identified and characterized in pheochromocytoma (PC12) cells using inside-out and cell-attached patch-clamp recordings. The channel shows a large unitary conductance (274 pS in symmetric 145 mM K+) and selectivity for Na+ ~ K+ > Li+, and is practically impermeable to Cl-. The channel activity-voltage relationship is hell-shaped, showing maximal activation at ~-10 mV. The overall activity of this channel is unmodified by [Na+](ic), or [Ca++](ic). However, increases in [Ca++](ic) lead to a decrease in the unitary current amplitude. In addition, overall activity is mildly increased when suction is applied to the back of the patch pipette. Together, these characteristics distinguish the present channel from all ether large conductance nonselective cation channels reported so far in a variety of preparations. The frequency of appearance of this channel type is similar in undifferentiated and NGF-treated PC12 cells (~8-27% of patches). The combination of large conductance, permeability to Na+, and existence of conducting states at negative potentials, may provide a significant pathway for inward current and depolarization in PC12 cells.

Original languageEnglish (US)
Pages (from-to)151-160
Number of pages10
JournalJournal of Membrane Biology
Volume160
Issue number2
DOIs
StatePublished - Dec 2 1997
Externally publishedYes

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PC12 Cells
Pheochromocytoma
Cations
Suction
Nerve Growth Factor
Ether
Permeability

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Physiology
  • Cell Biology

Cite this

A novel large conductance, nonselective cation channel in pheochromocytoma (PC12) cells. / Dopico, Alejandro; Treistman, S. N.

In: Journal of Membrane Biology, Vol. 160, No. 2, 02.12.1997, p. 151-160.

Research output: Contribution to journalArticle

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