A plasticizer released from IV drip chambers elevates calcium levels in neurosecretory terminals

K. Tully, D. Kupfer, Alejandro Dopico, S. N. Treistman

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We report that intracellular calcium levels rise in mammalian neurosecretory terminals and in cultured pheochromocytoma cells during acute exposure to physiological medium incubated in IV drip chambers. The agent responsible for this effect is shown to be di(2-ethylhexyl)phthalate (DEHP). DEHP (800 nM) added to saline solution caused a rise in [Ca2+](i) similar to that elicited by the contaminant-containing solution. The extraction of this contaminant from the IV drip chamber, as measured by spectrophotometry, was time-dependent and was markedly accelerated by the presence of 50 mM ethanol in the solution. Larger [Ca2+](i) increases were observed in terminals exposed to solutions incubated in IV drip chambers for greater durations. The rise in calcium requires transmembrane calcium flux through membrane channels, as the response is blocked by either 100 μM cadmium or by lowering the extracellular free Ca2+ concentration to 10 μM. Our results suggest that acute alterations in intracellular calcium should be considered in addition to long-term effects when determining the safety of phthalate-containing plastics and that laboratory researchers using plastic perfusion materials consider this potential source of artifactual results. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)183-188
Number of pages6
JournalToxicology and Applied Pharmacology
Volume168
Issue number3
DOIs
StatePublished - Nov 1 2000

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Plasticizers
Calcium
Plastics
Impurities
Spectrophotometry
Pheochromocytoma
Cadmium
Ion Channels
Sodium Chloride
Cultured Cells
Ethanol
Perfusion
Research Personnel
Fluxes
Safety
phthalic acid

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Pharmacology

Cite this

A plasticizer released from IV drip chambers elevates calcium levels in neurosecretory terminals. / Tully, K.; Kupfer, D.; Dopico, Alejandro; Treistman, S. N.

In: Toxicology and Applied Pharmacology, Vol. 168, No. 3, 01.11.2000, p. 183-188.

Research output: Contribution to journalArticle

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