A preliminary evaluation of glial cell line-derived neurotrophic factor (GDNF) levels in cerebrospinal fluid across various gestational ages and clinical conditions of the neonate

Rahul Rajkumar, Bhavana Bhaya, Divya Mamilla, Theresa Czech, Esther Kisseih, Arun Saini, Nitin Chouthai

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background This study aims to investigate glial cell derived neurotrophic factor (GDNF) levels in newborns’ umbilical cord blood and cerebrospinal fluid across various perinatal growth parameters and clinical conditions. Methods Cord blood from 20 newborns and 58 residual CSF samples (stored after completion of clinical testing) were collected. GDNF levels were determined using GDNF ELISA kits from R&D Systems in triplicates with appropriate controls to eliminate background. Results Cord blood GDNF levels were significantly higher (p = 0.004) in preterm newborns (n = 6) (115.05 ± 57.17, pg/ml) when compared to term newborns (n = 14) (19.67 ± 10.67, pg/ml). GDNF levels in CSF trended (p = 0.07) higher in term newborns (n = 10) (19.56 ± 9.11, pg/ml) when compared to preterm newborns at term or post term corrected gestational ages (n = 5) (14.49 ± 3.53, pg/ml). Conclusions GDNF levels in preterm newborns were higher in cord blood and lower in CSF as compared to term newborns. It is important to further study circulating and CSF-GDNF levels in newborns at different gestational ages and clinical conditions.

Original languageEnglish (US)
Pages (from-to)61-65
Number of pages5
JournalInternational Journal of Developmental Neuroscience
Volume65
DOIs
StatePublished - Feb 1 2018

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Glial Cell Line-Derived Neurotrophic Factor
Gestational Age
Cerebrospinal Fluid
Fetal Blood
Nerve Growth Factors
Neuroglia
Enzyme-Linked Immunosorbent Assay
Growth

All Science Journal Classification (ASJC) codes

  • Developmental Neuroscience
  • Developmental Biology

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A preliminary evaluation of glial cell line-derived neurotrophic factor (GDNF) levels in cerebrospinal fluid across various gestational ages and clinical conditions of the neonate. / Rajkumar, Rahul; Bhaya, Bhavana; Mamilla, Divya; Czech, Theresa; Kisseih, Esther; Saini, Arun; Chouthai, Nitin.

In: International Journal of Developmental Neuroscience, Vol. 65, 01.02.2018, p. 61-65.

Research output: Contribution to journalArticle

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abstract = "Background This study aims to investigate glial cell derived neurotrophic factor (GDNF) levels in newborns’ umbilical cord blood and cerebrospinal fluid across various perinatal growth parameters and clinical conditions. Methods Cord blood from 20 newborns and 58 residual CSF samples (stored after completion of clinical testing) were collected. GDNF levels were determined using GDNF ELISA kits from R&D Systems in triplicates with appropriate controls to eliminate background. Results Cord blood GDNF levels were significantly higher (p = 0.004) in preterm newborns (n = 6) (115.05 ± 57.17, pg/ml) when compared to term newborns (n = 14) (19.67 ± 10.67, pg/ml). GDNF levels in CSF trended (p = 0.07) higher in term newborns (n = 10) (19.56 ± 9.11, pg/ml) when compared to preterm newborns at term or post term corrected gestational ages (n = 5) (14.49 ± 3.53, pg/ml). Conclusions GDNF levels in preterm newborns were higher in cord blood and lower in CSF as compared to term newborns. It is important to further study circulating and CSF-GDNF levels in newborns at different gestational ages and clinical conditions.",
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AU - Czech, Theresa

AU - Kisseih, Esther

AU - Saini, Arun

AU - Chouthai, Nitin

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