A prostate-derived cDNA that is mapped to human chromosome 19 encodes a novel protein

Chiang Wang, Ida M. McCarty, Louisa Balazs, Yi Li, Mitchell S. Steiner

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The epithelial cells of prostate gland secrete various secretory products that play an important role in the growth and differentiation of prostate gland. These secretory products have also been implicated in neuroendocrine differentiation of benign prostatic hyperplasia and prostate malignancy. We have cloned a prostate-derived cDNA encoding a novel protein with a predicted molecular weight of 78 kDa (P78), and precisely mapped the cDNA sequence to chromosome 19. The P78 gene has a complex genomic structure with 18 exons and 17 introns. The P78 contains two conserved structural domains with limited similarity to domain D of synapsin I. The P78 mRNA was expressed in various human cell lines. Western blot analysis using antibody specific for the P78 revealed the presence of the P78 protein in the prostate cancer cell lines with much lower level in metastatic prostate cancer cell lines compared to that in a primary prostate cancer cell line.

Original languageEnglish (US)
Pages (from-to)281-287
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume296
Issue number2
DOIs
StatePublished - Oct 14 2002

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Chromosomes, Human, Pair 19
Human Chromosomes
Chromosomes
Prostate
Complementary DNA
Cells
Cell Line
Prostatic Neoplasms
Proteins
Synapsins
Prostatic Hyperplasia
Introns
Exons
Genes
Molecular Weight
Western Blotting
Epithelial Cells
Molecular weight
Messenger RNA
Antibodies

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

A prostate-derived cDNA that is mapped to human chromosome 19 encodes a novel protein. / Wang, Chiang; McCarty, Ida M.; Balazs, Louisa; Li, Yi; Steiner, Mitchell S.

In: Biochemical and Biophysical Research Communications, Vol. 296, No. 2, 14.10.2002, p. 281-287.

Research output: Contribution to journalArticle

Wang, Chiang ; McCarty, Ida M. ; Balazs, Louisa ; Li, Yi ; Steiner, Mitchell S. / A prostate-derived cDNA that is mapped to human chromosome 19 encodes a novel protein. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 296, No. 2. pp. 281-287.
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