A quantitative assay for IgA rheumatoid factor

William J. Koopman, Ralph E. Schrohenloher, Alan Solomon

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We have developed a solid-phase radioimmunoassay capable of detecting nanogram quantities of human IgA rheumatoid factor (RF) in biological fluids. Human IgM RF, IgG RF, IgG, IgA, IgM and whole serum did not significantly interfere with the IgA RF assay. Patients with sero-positive rheumatoid arthritis (RA) had significantly higher concentrations of IgA RF than sero-negative RA patients or healthy adult controls. Concentrations of IgA RF in paired sera and synovial fluids from sero-positive RA patients were comparable. Levels of IgA RF demonstrated a moderately good correlation with levels of IgM RF in sero-positive RA sera (r = 0.673). However, the ratio of IgA RF concentration to IgM RF concentration in sero-positive RA sera varied widely.

Original languageEnglish (US)
Pages (from-to)89-98
Number of pages10
JournalJournal of Immunological Methods
Volume50
Issue number1
DOIs
StatePublished - Apr 16 1982

Fingerprint

Rheumatoid Factor
Immunoglobulin A
Rheumatoid Arthritis
Immunoglobulin M
Serum
Immunoglobulin G
Synovial Fluid
Radioimmunoassay

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

A quantitative assay for IgA rheumatoid factor. / Koopman, William J.; Schrohenloher, Ralph E.; Solomon, Alan.

In: Journal of Immunological Methods, Vol. 50, No. 1, 16.04.1982, p. 89-98.

Research output: Contribution to journalArticle

Koopman, William J. ; Schrohenloher, Ralph E. ; Solomon, Alan. / A quantitative assay for IgA rheumatoid factor. In: Journal of Immunological Methods. 1982 ; Vol. 50, No. 1. pp. 89-98.
@article{52b8c8b0774f4ab08763e90a56f07a18,
title = "A quantitative assay for IgA rheumatoid factor",
abstract = "We have developed a solid-phase radioimmunoassay capable of detecting nanogram quantities of human IgA rheumatoid factor (RF) in biological fluids. Human IgM RF, IgG RF, IgG, IgA, IgM and whole serum did not significantly interfere with the IgA RF assay. Patients with sero-positive rheumatoid arthritis (RA) had significantly higher concentrations of IgA RF than sero-negative RA patients or healthy adult controls. Concentrations of IgA RF in paired sera and synovial fluids from sero-positive RA patients were comparable. Levels of IgA RF demonstrated a moderately good correlation with levels of IgM RF in sero-positive RA sera (r = 0.673). However, the ratio of IgA RF concentration to IgM RF concentration in sero-positive RA sera varied widely.",
author = "Koopman, {William J.} and Schrohenloher, {Ralph E.} and Alan Solomon",
year = "1982",
month = "4",
day = "16",
doi = "10.1016/0022-1759(82)90306-4",
language = "English (US)",
volume = "50",
pages = "89--98",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - A quantitative assay for IgA rheumatoid factor

AU - Koopman, William J.

AU - Schrohenloher, Ralph E.

AU - Solomon, Alan

PY - 1982/4/16

Y1 - 1982/4/16

N2 - We have developed a solid-phase radioimmunoassay capable of detecting nanogram quantities of human IgA rheumatoid factor (RF) in biological fluids. Human IgM RF, IgG RF, IgG, IgA, IgM and whole serum did not significantly interfere with the IgA RF assay. Patients with sero-positive rheumatoid arthritis (RA) had significantly higher concentrations of IgA RF than sero-negative RA patients or healthy adult controls. Concentrations of IgA RF in paired sera and synovial fluids from sero-positive RA patients were comparable. Levels of IgA RF demonstrated a moderately good correlation with levels of IgM RF in sero-positive RA sera (r = 0.673). However, the ratio of IgA RF concentration to IgM RF concentration in sero-positive RA sera varied widely.

AB - We have developed a solid-phase radioimmunoassay capable of detecting nanogram quantities of human IgA rheumatoid factor (RF) in biological fluids. Human IgM RF, IgG RF, IgG, IgA, IgM and whole serum did not significantly interfere with the IgA RF assay. Patients with sero-positive rheumatoid arthritis (RA) had significantly higher concentrations of IgA RF than sero-negative RA patients or healthy adult controls. Concentrations of IgA RF in paired sera and synovial fluids from sero-positive RA patients were comparable. Levels of IgA RF demonstrated a moderately good correlation with levels of IgM RF in sero-positive RA sera (r = 0.673). However, the ratio of IgA RF concentration to IgM RF concentration in sero-positive RA sera varied widely.

UR - http://www.scopus.com/inward/record.url?scp=0020027416&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020027416&partnerID=8YFLogxK

U2 - 10.1016/0022-1759(82)90306-4

DO - 10.1016/0022-1759(82)90306-4

M3 - Article

C2 - 7086151

AN - SCOPUS:0020027416

VL - 50

SP - 89

EP - 98

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 1

ER -