A quantitative genetic analysis of slow-wave sleep in influenza-infected CXB recombinant inbred mice

L. A. Toth, Robert Williams

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Influenza-infected C57BL/6J mice spend increased amounts of time in slow-wave sleep (SWS) during the dark phase of the circadian cycle compared to healthy mice. In contrast, infected BALB/cByJ mice show a normal or reduced time in SWS, particularly during the light phase. To identify genetic loci with linkage to these traits, we measured sleep in 13 CXB recombinant inbred (RI) strains derived from a cross between C57BL/6ByJ and BALB/cByJ mice. The probability density distribution of sleep patterns of influenza- infected CXB RI mice showed modes that correspond roughly with the parental modes during the dark phase of the circadian cycle and are intermediate or C57BL/6-like during the light phase. These patterns are consistent with the presence of a low number of major effect quantitative trait loci (QTLs). Chromosomal regions with provisional association to strain variation in influenza-induced SWS patterns were identified. In particular, a 10- to 12-cM interval on Chr 6 between D6Mit74 and D6Mit188 contains a QTL (LRS = 16.6 at 1 cM proximal to D6Mit316; genomewide p < .05) that influences the SWS response to influenza infection during the light phase. We have provisionally named this QTL Srilp1 (sleep response to influenza, light phase 1). Candidate genes for mediation of this phenotype include Ghrhr (growth hormone releasing hormone receptor), Crhr2 (corticotropin releasing hormone receptor 2), and Cd8a (an epitope on cytotoxic T lymphocytes). Several other intervals achieved suggestive probability scores that are sufficient to warrant further analysis either with additional RI strains or with F2 panels. The analysis also suggests that dark phase and light phase responses are regulated by different genetic factors.

Original languageEnglish (US)
Pages (from-to)339-348
Number of pages10
JournalBehavior Genetics
Volume29
Issue number5
DOIs
StatePublished - Jan 1 1999

Fingerprint

influenza
sleep
quantitative genetics
genetic analysis
Human Influenza
genetic techniques and protocols
Sleep
mice
Quantitative Trait Loci
Light
hormone
quantitative trait loci
hormone receptors
Corticotropin-Releasing Hormone Receptors
somatoliberin
corticotropin-releasing hormone
cytotoxic T-lymphocytes
Genetic Loci
strain differences
Cytotoxic T-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Genetics(clinical)

Cite this

A quantitative genetic analysis of slow-wave sleep in influenza-infected CXB recombinant inbred mice. / Toth, L. A.; Williams, Robert.

In: Behavior Genetics, Vol. 29, No. 5, 01.01.1999, p. 339-348.

Research output: Contribution to journalArticle

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