A rat monoclonal antibody specific for murine type 1 pneumocytes

Jon A. Hotchkiss, Stephen Kennel, Jack R. Harkema

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A rat monoclonal antibody (MAb), 411-52, that binds specifically to murine pulmonary alveolar type 1 cells was developed. The cell-binding specificity of MAb 411-52 was assessed by light microscopy on immunoperoxidase-labeled tissue sections, electron microscopy on immunogold-labeled tissue blocks, and by flow cytometric analysis and fluorescence-activated cell sorting of immunofluorescently labeled cells enzymatically dissociated from murine lungs. The epitope recognized by MAb 411-52 was first detected in immunoperoxidase-stained sections of neonatal lungs of mice approximately 3 weeks after birth. In adult mice, the MAb 411-52-directed, immunoperoxidase-staining pattern was uniform throughout the lung parenchyma, was restricted to the luminal surfaces of alveoli, and was absent from type 2, endothelial, and interstitial cells, as well as from the epithelial cells of conducting airways. Electron microscopic analysis of immunogold-labeled lung tissue confirmed the type 1 cell binding specificity of MAb 411-52. Analysis by multiparameter, laser flow cytometry indicated that MAb 411-52 binds to 4.6 ± 0.5% (mean ± SD) of enzymatically dissociated cells from the lungs of normal adult mice. The absence of immunogold-labeling of type 2 cells suggested that the epitope recognized by MAb 411-52 might be a differentiation marker for the type 1 cell phenotype. With this MAb and standard immunohistochemical techniques, it is possible to visualize directly type 1 cells in paraffin sections.

Original languageEnglish (US)
Pages (from-to)235-246
Number of pages12
JournalExperimental and Molecular Pathology
Volume57
Issue number3
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

Fingerprint

Alveolar Epithelial Cells
Rats
Monoclonal Antibodies
Lung
Tissue
Epitopes
Flow Cytometry
Flow cytometry
Differentiation Antigens
Sorting
Paraffin
Labeling
Electron microscopy
Optical microscopy
Microscopy
Electron Microscopy
Lasers
Endothelial Cells
Epithelial Cells
Fluorescence

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Molecular Biology
  • Pathology and Forensic Medicine

Cite this

A rat monoclonal antibody specific for murine type 1 pneumocytes. / Hotchkiss, Jon A.; Kennel, Stephen; Harkema, Jack R.

In: Experimental and Molecular Pathology, Vol. 57, No. 3, 01.01.1992, p. 235-246.

Research output: Contribution to journalArticle

Hotchkiss, Jon A. ; Kennel, Stephen ; Harkema, Jack R. / A rat monoclonal antibody specific for murine type 1 pneumocytes. In: Experimental and Molecular Pathology. 1992 ; Vol. 57, No. 3. pp. 235-246.
@article{ea5be91e438943cfa502ae57459f9fd8,
title = "A rat monoclonal antibody specific for murine type 1 pneumocytes",
abstract = "A rat monoclonal antibody (MAb), 411-52, that binds specifically to murine pulmonary alveolar type 1 cells was developed. The cell-binding specificity of MAb 411-52 was assessed by light microscopy on immunoperoxidase-labeled tissue sections, electron microscopy on immunogold-labeled tissue blocks, and by flow cytometric analysis and fluorescence-activated cell sorting of immunofluorescently labeled cells enzymatically dissociated from murine lungs. The epitope recognized by MAb 411-52 was first detected in immunoperoxidase-stained sections of neonatal lungs of mice approximately 3 weeks after birth. In adult mice, the MAb 411-52-directed, immunoperoxidase-staining pattern was uniform throughout the lung parenchyma, was restricted to the luminal surfaces of alveoli, and was absent from type 2, endothelial, and interstitial cells, as well as from the epithelial cells of conducting airways. Electron microscopic analysis of immunogold-labeled lung tissue confirmed the type 1 cell binding specificity of MAb 411-52. Analysis by multiparameter, laser flow cytometry indicated that MAb 411-52 binds to 4.6 ± 0.5{\%} (mean ± SD) of enzymatically dissociated cells from the lungs of normal adult mice. The absence of immunogold-labeling of type 2 cells suggested that the epitope recognized by MAb 411-52 might be a differentiation marker for the type 1 cell phenotype. With this MAb and standard immunohistochemical techniques, it is possible to visualize directly type 1 cells in paraffin sections.",
author = "Hotchkiss, {Jon A.} and Stephen Kennel and Harkema, {Jack R.}",
year = "1992",
month = "1",
day = "1",
doi = "10.1016/0014-4800(92)90014-3",
language = "English (US)",
volume = "57",
pages = "235--246",
journal = "Experimental and Molecular Pathology",
issn = "0014-4800",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - A rat monoclonal antibody specific for murine type 1 pneumocytes

AU - Hotchkiss, Jon A.

AU - Kennel, Stephen

AU - Harkema, Jack R.

PY - 1992/1/1

Y1 - 1992/1/1

N2 - A rat monoclonal antibody (MAb), 411-52, that binds specifically to murine pulmonary alveolar type 1 cells was developed. The cell-binding specificity of MAb 411-52 was assessed by light microscopy on immunoperoxidase-labeled tissue sections, electron microscopy on immunogold-labeled tissue blocks, and by flow cytometric analysis and fluorescence-activated cell sorting of immunofluorescently labeled cells enzymatically dissociated from murine lungs. The epitope recognized by MAb 411-52 was first detected in immunoperoxidase-stained sections of neonatal lungs of mice approximately 3 weeks after birth. In adult mice, the MAb 411-52-directed, immunoperoxidase-staining pattern was uniform throughout the lung parenchyma, was restricted to the luminal surfaces of alveoli, and was absent from type 2, endothelial, and interstitial cells, as well as from the epithelial cells of conducting airways. Electron microscopic analysis of immunogold-labeled lung tissue confirmed the type 1 cell binding specificity of MAb 411-52. Analysis by multiparameter, laser flow cytometry indicated that MAb 411-52 binds to 4.6 ± 0.5% (mean ± SD) of enzymatically dissociated cells from the lungs of normal adult mice. The absence of immunogold-labeling of type 2 cells suggested that the epitope recognized by MAb 411-52 might be a differentiation marker for the type 1 cell phenotype. With this MAb and standard immunohistochemical techniques, it is possible to visualize directly type 1 cells in paraffin sections.

AB - A rat monoclonal antibody (MAb), 411-52, that binds specifically to murine pulmonary alveolar type 1 cells was developed. The cell-binding specificity of MAb 411-52 was assessed by light microscopy on immunoperoxidase-labeled tissue sections, electron microscopy on immunogold-labeled tissue blocks, and by flow cytometric analysis and fluorescence-activated cell sorting of immunofluorescently labeled cells enzymatically dissociated from murine lungs. The epitope recognized by MAb 411-52 was first detected in immunoperoxidase-stained sections of neonatal lungs of mice approximately 3 weeks after birth. In adult mice, the MAb 411-52-directed, immunoperoxidase-staining pattern was uniform throughout the lung parenchyma, was restricted to the luminal surfaces of alveoli, and was absent from type 2, endothelial, and interstitial cells, as well as from the epithelial cells of conducting airways. Electron microscopic analysis of immunogold-labeled lung tissue confirmed the type 1 cell binding specificity of MAb 411-52. Analysis by multiparameter, laser flow cytometry indicated that MAb 411-52 binds to 4.6 ± 0.5% (mean ± SD) of enzymatically dissociated cells from the lungs of normal adult mice. The absence of immunogold-labeling of type 2 cells suggested that the epitope recognized by MAb 411-52 might be a differentiation marker for the type 1 cell phenotype. With this MAb and standard immunohistochemical techniques, it is possible to visualize directly type 1 cells in paraffin sections.

UR - http://www.scopus.com/inward/record.url?scp=0027053817&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027053817&partnerID=8YFLogxK

U2 - 10.1016/0014-4800(92)90014-3

DO - 10.1016/0014-4800(92)90014-3

M3 - Article

VL - 57

SP - 235

EP - 246

JO - Experimental and Molecular Pathology

JF - Experimental and Molecular Pathology

SN - 0014-4800

IS - 3

ER -