A self-organising biomimetic collagen/nano-hydroxyapatite-glycosaminoglycan scaffold for spinal fusion

Aman Sharma, David Brand, Jeremy Fairbank, Hua Ye, Chris Lavy, Jan Czernuszka

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The use of spinal fusion surgery as a treatment for degenerative spinal conditions and chronic back pain is increasing. However, this technique requires use of a bone grafting material to fuse the vertebrae, traditionally autologous bone, which consists of an optimal combination of osteogenic cell precursors, extracellular matrix proteins and mineral components. To date, this remains the ‘gold standard’ material but its supply is limited and is associated with a number of clinical and ethical difficulties; consequently, various combinations of cells with biological scaffold materials have been tested but have failed to achieve fusion rates even comparable to autologous bone. We successfully fabricated a novel collagen-based scaffold using self-organising atelocollagen combined with nano-hydroxyapatite and chondroitin sulphate, cross-linked by microbial transglutaminase. The scaffold was characterised using a range of imaging, chemical composition and thermal analysis techniques. It was found to exhibit appropriate stiffness and suitable pore size for the adhesion, growth and differentiation of MSCs. The low toxicity makes it suitable for clinical application, and its slow degradation profile would enable the scaffold to promote bone growth over an extended period. This material therefore shows promise for clinical use in spinal fusion and other procedures requiring the use of bone grafts.

Original languageEnglish (US)
Pages (from-to)12574-12592
Number of pages19
JournalJournal of Materials Science
Volume52
Issue number21
DOIs
StatePublished - Nov 1 2017

Fingerprint

Biomimetics
Scaffolds (biology)
Glycosaminoglycans
Hydroxyapatite
Collagen
Bone
Fusion reactions
Scaffolds
Transglutaminases
Chondroitin Sulfates
Extracellular Matrix Proteins
Electric fuses
Durapatite
Grafts
Surgery
Thermoanalysis
Pore size
Minerals
Toxicity
Adhesion

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

A self-organising biomimetic collagen/nano-hydroxyapatite-glycosaminoglycan scaffold for spinal fusion. / Sharma, Aman; Brand, David; Fairbank, Jeremy; Ye, Hua; Lavy, Chris; Czernuszka, Jan.

In: Journal of Materials Science, Vol. 52, No. 21, 01.11.2017, p. 12574-12592.

Research output: Contribution to journalArticle

Sharma, Aman ; Brand, David ; Fairbank, Jeremy ; Ye, Hua ; Lavy, Chris ; Czernuszka, Jan. / A self-organising biomimetic collagen/nano-hydroxyapatite-glycosaminoglycan scaffold for spinal fusion. In: Journal of Materials Science. 2017 ; Vol. 52, No. 21. pp. 12574-12592.
@article{3b1497ab50c6433797bb6b05fa132629,
title = "A self-organising biomimetic collagen/nano-hydroxyapatite-glycosaminoglycan scaffold for spinal fusion",
abstract = "The use of spinal fusion surgery as a treatment for degenerative spinal conditions and chronic back pain is increasing. However, this technique requires use of a bone grafting material to fuse the vertebrae, traditionally autologous bone, which consists of an optimal combination of osteogenic cell precursors, extracellular matrix proteins and mineral components. To date, this remains the ‘gold standard’ material but its supply is limited and is associated with a number of clinical and ethical difficulties; consequently, various combinations of cells with biological scaffold materials have been tested but have failed to achieve fusion rates even comparable to autologous bone. We successfully fabricated a novel collagen-based scaffold using self-organising atelocollagen combined with nano-hydroxyapatite and chondroitin sulphate, cross-linked by microbial transglutaminase. The scaffold was characterised using a range of imaging, chemical composition and thermal analysis techniques. It was found to exhibit appropriate stiffness and suitable pore size for the adhesion, growth and differentiation of MSCs. The low toxicity makes it suitable for clinical application, and its slow degradation profile would enable the scaffold to promote bone growth over an extended period. This material therefore shows promise for clinical use in spinal fusion and other procedures requiring the use of bone grafts.",
author = "Aman Sharma and David Brand and Jeremy Fairbank and Hua Ye and Chris Lavy and Jan Czernuszka",
year = "2017",
month = "11",
day = "1",
doi = "10.1007/s10853-017-1229-9",
language = "English (US)",
volume = "52",
pages = "12574--12592",
journal = "Journal of Materials Science",
issn = "0022-2461",
publisher = "Springer Netherlands",
number = "21",

}

TY - JOUR

T1 - A self-organising biomimetic collagen/nano-hydroxyapatite-glycosaminoglycan scaffold for spinal fusion

AU - Sharma, Aman

AU - Brand, David

AU - Fairbank, Jeremy

AU - Ye, Hua

AU - Lavy, Chris

AU - Czernuszka, Jan

PY - 2017/11/1

Y1 - 2017/11/1

N2 - The use of spinal fusion surgery as a treatment for degenerative spinal conditions and chronic back pain is increasing. However, this technique requires use of a bone grafting material to fuse the vertebrae, traditionally autologous bone, which consists of an optimal combination of osteogenic cell precursors, extracellular matrix proteins and mineral components. To date, this remains the ‘gold standard’ material but its supply is limited and is associated with a number of clinical and ethical difficulties; consequently, various combinations of cells with biological scaffold materials have been tested but have failed to achieve fusion rates even comparable to autologous bone. We successfully fabricated a novel collagen-based scaffold using self-organising atelocollagen combined with nano-hydroxyapatite and chondroitin sulphate, cross-linked by microbial transglutaminase. The scaffold was characterised using a range of imaging, chemical composition and thermal analysis techniques. It was found to exhibit appropriate stiffness and suitable pore size for the adhesion, growth and differentiation of MSCs. The low toxicity makes it suitable for clinical application, and its slow degradation profile would enable the scaffold to promote bone growth over an extended period. This material therefore shows promise for clinical use in spinal fusion and other procedures requiring the use of bone grafts.

AB - The use of spinal fusion surgery as a treatment for degenerative spinal conditions and chronic back pain is increasing. However, this technique requires use of a bone grafting material to fuse the vertebrae, traditionally autologous bone, which consists of an optimal combination of osteogenic cell precursors, extracellular matrix proteins and mineral components. To date, this remains the ‘gold standard’ material but its supply is limited and is associated with a number of clinical and ethical difficulties; consequently, various combinations of cells with biological scaffold materials have been tested but have failed to achieve fusion rates even comparable to autologous bone. We successfully fabricated a novel collagen-based scaffold using self-organising atelocollagen combined with nano-hydroxyapatite and chondroitin sulphate, cross-linked by microbial transglutaminase. The scaffold was characterised using a range of imaging, chemical composition and thermal analysis techniques. It was found to exhibit appropriate stiffness and suitable pore size for the adhesion, growth and differentiation of MSCs. The low toxicity makes it suitable for clinical application, and its slow degradation profile would enable the scaffold to promote bone growth over an extended period. This material therefore shows promise for clinical use in spinal fusion and other procedures requiring the use of bone grafts.

UR - http://www.scopus.com/inward/record.url?scp=85026458315&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85026458315&partnerID=8YFLogxK

U2 - 10.1007/s10853-017-1229-9

DO - 10.1007/s10853-017-1229-9

M3 - Article

VL - 52

SP - 12574

EP - 12592

JO - Journal of Materials Science

JF - Journal of Materials Science

SN - 0022-2461

IS - 21

ER -