A sensitive LC-MS/MS method for the quantification of urinary 8-iso-prostaglandin F (8-iso-PGF ) including pediatric reference interval

Yi Xiao, Xiaowei Fu, Paul Pattengale, Jennifer Dien Bard, Yan Kang Xu, Maurice R. O'Gorman

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background Oxidative stress has been implicated in numerous diseases, including arthritis, atherosclerosis, Alzheimer's disease, cancer, diabetes, hypertension, and inflammation. 8-iso-prostaglandin F , a member of the F2 isoprostane family, has been well-accepted as a valuable biomarker for the assessment of oxidative stress. Methods We report the development and validation of an ultra-sensitive LC-MS/MS assay for urinary 8-iso-PGF measurements in pediatric population. Results The assay was linear from 0.024 to 20 nmol/l (R 2  = 0.99). Recoveries were above 85% and matrix effects were below 5%. The variability was determined at nmol/l concentration: the intra-day variability (%CV) ranged from 3.9% to 4.5% (n = 20); and the inter-day variability ranged from 4.3% to 5.7% (n = 20). The accuracy of our laboratory developed test was evaluated with a clinical reference laboratory (n = 39), and a correlation coefficient of 0.9257 was observed. Reference interval were established to be < 0.5 ng/mg creatinine in a group of pediatric population (2 months–18 years, n = 123). Conclusions The precision of the assay will allow for accurate assessment of oxidative stress, and is acceptable for patient testing, particularly in pediatric population.

Original languageEnglish (US)
Pages (from-to)128-134
Number of pages7
JournalClinica Chimica Acta
Volume460
DOIs
StatePublished - Sep 1 2016

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Pediatrics
Oxidative stress
Prostaglandins F
Assays
Oxidative Stress
F2-Isoprostanes
Biomarkers
Medical problems
Population Groups
Population
Arthritis
Creatinine
Atherosclerosis
Alzheimer Disease
Hypertension
Inflammation
Recovery
Testing
Neoplasms

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

A sensitive LC-MS/MS method for the quantification of urinary 8-iso-prostaglandin F (8-iso-PGF ) including pediatric reference interval . / Xiao, Yi; Fu, Xiaowei; Pattengale, Paul; Dien Bard, Jennifer; Xu, Yan Kang; O'Gorman, Maurice R.

In: Clinica Chimica Acta, Vol. 460, 01.09.2016, p. 128-134.

Research output: Contribution to journalArticle

Xiao, Yi ; Fu, Xiaowei ; Pattengale, Paul ; Dien Bard, Jennifer ; Xu, Yan Kang ; O'Gorman, Maurice R. / A sensitive LC-MS/MS method for the quantification of urinary 8-iso-prostaglandin F (8-iso-PGF ) including pediatric reference interval In: Clinica Chimica Acta. 2016 ; Vol. 460. pp. 128-134.
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abstract = "Background Oxidative stress has been implicated in numerous diseases, including arthritis, atherosclerosis, Alzheimer's disease, cancer, diabetes, hypertension, and inflammation. 8-iso-prostaglandin F 2α , a member of the F2 isoprostane family, has been well-accepted as a valuable biomarker for the assessment of oxidative stress. Methods We report the development and validation of an ultra-sensitive LC-MS/MS assay for urinary 8-iso-PGF 2α measurements in pediatric population. Results The assay was linear from 0.024 to 20 nmol/l (R 2  = 0.99). Recoveries were above 85{\%} and matrix effects were below 5{\%}. The variability was determined at nmol/l concentration: the intra-day variability ({\%}CV) ranged from 3.9{\%} to 4.5{\%} (n = 20); and the inter-day variability ranged from 4.3{\%} to 5.7{\%} (n = 20). The accuracy of our laboratory developed test was evaluated with a clinical reference laboratory (n = 39), and a correlation coefficient of 0.9257 was observed. Reference interval were established to be < 0.5 ng/mg creatinine in a group of pediatric population (2 months–18 years, n = 123). Conclusions The precision of the assay will allow for accurate assessment of oxidative stress, and is acceptable for patient testing, particularly in pediatric population.",
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