A silica-based mitochondrial DNA extraction method applied to forensic hair shafts and teeth

L. E. Baker, W. F. McCormick, Karla Matteson

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The purpose of this study is to evaluate the applicability of a nonorganic DNA extraction method for use in the analysis of environmentally compromised forensic hair shaft and tooth samples. The condition of the samples included cases of water decomposition, severe incineration, and varying stages of putrefaction. Enzymatic amplification and manual sequencing of the first segment of the mitochondrial hypervariable region were performed successfully on each of the 20 autopsied individuals. The results indicate that the silica-based extraction method produces mtDNA suitable for genetic identification from forensic samples including hair shafts and teeth.

Original languageEnglish (US)
Pages (from-to)126-130
Number of pages5
JournalJournal of Forensic Sciences
Volume46
Issue number1
StatePublished - Mar 1 2001
Externally publishedYes

Fingerprint

Mitochondrial DNA
Silicon Dioxide
Hair
Tooth
Forensic Genetics
Incineration
Water
DNA

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Genetics

Cite this

A silica-based mitochondrial DNA extraction method applied to forensic hair shafts and teeth. / Baker, L. E.; McCormick, W. F.; Matteson, Karla.

In: Journal of Forensic Sciences, Vol. 46, No. 1, 01.03.2001, p. 126-130.

Research output: Contribution to journalArticle

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