A single amino acid change in the C-terminal domain of the matrix protein M1 of influenza B virus confers mouse adaptation and virulence

Jonathan Mccullers, Erich Hoffmann, Victor C. Huber, Asia D. Nickerson

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Serial passage of an initially avirulent influenza B virus, B/Memphis/12/97, resulted in the selection of a variant which was lethal in mice. Virulence correlated with improved growth in vivo and prolonged replication. Sequencing of the complete coding regions of the parent and mouse-adapted viruses revealed 8 amino acid differences. Sequencing and characterization of intermediate passages suggested that one change in the C-terminal domain of the M1 protein, an asparagine to a serine at position 221, was responsible for acquisition of virulence and lethality. Site-directed mutagenesis of the M segment of a different virus, B/Yamanashi/166/98, to change this amino acid residue confirmed its importance by conferring improved growth and virulence in mice. This observation suggests a role for the C domain of the M1 protein in growth and virulence in a mammalian host.

Original languageEnglish (US)
Pages (from-to)318-326
Number of pages9
JournalVirology
Volume336
Issue number2
DOIs
StatePublished - Jun 5 2005

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Virulence
Amino Acids
Growth
Influenza B virus
Serial Passage
Cercopithecine Herpesvirus 1
Asparagine
Site-Directed Mutagenesis
Serine
Viruses
influenza B virus M1 matrix protein
Protein Domains

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

A single amino acid change in the C-terminal domain of the matrix protein M1 of influenza B virus confers mouse adaptation and virulence. / Mccullers, Jonathan; Hoffmann, Erich; Huber, Victor C.; Nickerson, Asia D.

In: Virology, Vol. 336, No. 2, 05.06.2005, p. 318-326.

Research output: Contribution to journalArticle

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