A spectrin-like protein from mouse brain membranes

phosphorylation of the 235,000-dalton subunit.

Steven Goodman, I. S. Zagon, C. F. Whitfield, L. A. Casoria, S. B. Shohet, S. E. Bernstein, P. J. McLaughlin, T. L. Laskiewicz

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A mouse brain spectrin-like protein, which was an immunoreactive analogue of erythrocyte spectrin, has been isolated from demyelinated membranes. This spectrin analogue was a 10.5 S, 972,000 molecular weight (Mr) (alpha beta)2 tetramer containing subunits of 240,000 (alpha) and 235,000 (beta) Mr. We demonstrated that in vivo only the 235,000 Mr beta subunit of the mouse brain spectrin-like protein was phosphorylated, which was an analogous situation to mouse erythrocyte spectrin in which only the 220,000 Mr beta subunit was phosphorylated. Incubation of isolated membrane fractions with [gamma-32P]ATP +/- adenosine 3',5'-cyclic monophosphate (cAMP) indicated that mouse brain spectrin-like protein, mouse erythrocyte spectrin, and human erythrocyte spectrin's beta subunits were all phosphorylated in vitro by membrane-associated cAMP-independent protein kinases.

Original languageEnglish (US)
JournalThe American journal of physiology
Volume247
Issue number1 Pt 1
StatePublished - Jul 1984
Externally publishedYes

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Spectrin
Phosphorylation
Erythrocytes
Membranes
Brain
Cyclic AMP
Protein Kinases
Adenosine Triphosphate
Molecular Weight
spectrin-like proteins

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Goodman, S., Zagon, I. S., Whitfield, C. F., Casoria, L. A., Shohet, S. B., Bernstein, S. E., ... Laskiewicz, T. L. (1984). A spectrin-like protein from mouse brain membranes: phosphorylation of the 235,000-dalton subunit. The American journal of physiology, 247(1 Pt 1).

A spectrin-like protein from mouse brain membranes : phosphorylation of the 235,000-dalton subunit. / Goodman, Steven; Zagon, I. S.; Whitfield, C. F.; Casoria, L. A.; Shohet, S. B.; Bernstein, S. E.; McLaughlin, P. J.; Laskiewicz, T. L.

In: The American journal of physiology, Vol. 247, No. 1 Pt 1, 07.1984.

Research output: Contribution to journalArticle

Goodman, S, Zagon, IS, Whitfield, CF, Casoria, LA, Shohet, SB, Bernstein, SE, McLaughlin, PJ & Laskiewicz, TL 1984, 'A spectrin-like protein from mouse brain membranes: phosphorylation of the 235,000-dalton subunit.', The American journal of physiology, vol. 247, no. 1 Pt 1.
Goodman, Steven ; Zagon, I. S. ; Whitfield, C. F. ; Casoria, L. A. ; Shohet, S. B. ; Bernstein, S. E. ; McLaughlin, P. J. ; Laskiewicz, T. L. / A spectrin-like protein from mouse brain membranes : phosphorylation of the 235,000-dalton subunit. In: The American journal of physiology. 1984 ; Vol. 247, No. 1 Pt 1.
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