A streamlined protocol for extracting RNA and genomic DNA from archived human blood and muscle

Gipsy Majumdar, Santiago Vera, Marshall Elam, Rajendra Raghow

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We combined the TRIzol method of nucleic acid extraction with QIAamp columns to achieve coextraction of RNA and genomic DNA from peripheral blood mononuclear cells (PBMCs) and biopsied skeletal muscle, both stored at -80 °C for many months. Total RNA was recovered from the upper aqueous phase of TRIzol. The interphase and organic phases were precipitated with ethanol, digested with proteinase K, and filtered through QIAamp MinElute columns to recover DNA. The combined protocol yielded excellent quality and quantity of nucleic acids from archived human PBMCs and muscle and may be easily adapted for other tissues.

Original languageEnglish (US)
Pages (from-to)25-27
Number of pages3
JournalAnalytical Biochemistry
Volume474
DOIs
StatePublished - Apr 1 2015

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Nucleic Acids
Muscle
Blood Cells
Blood
RNA
Muscles
Endopeptidase K
DNA
Interphase
Skeletal Muscle
Ethanol
Tissue
trizol

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

A streamlined protocol for extracting RNA and genomic DNA from archived human blood and muscle. / Majumdar, Gipsy; Vera, Santiago; Elam, Marshall; Raghow, Rajendra.

In: Analytical Biochemistry, Vol. 474, 01.04.2015, p. 25-27.

Research output: Contribution to journalArticle

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