A survey of potential problems and quality control in peptide synthesis by the fluorenylmethoxycarbonyl procedure.

J. D. Fontenot, J. M. Ball, Mark Miller, C. M. David, R. C. Montelaro

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The routine production of peptides by manual or automated solid-phase synthesis protocols has gained widespread usage among a variety of biological scientists as new synthetic procedures have been introduced over the past several years. We report here a detailed analysis of Fmoc synthesis procedures to identify problematic reactions and to evaluate analytical procedures for monitoring the quality of peptides during and after synthesis. The results of these studies demonstrate double additions of particular amino acids during single coupling cycles, frequent incomplete deblocking of peptides by standard piperidine reactions, and the failure of the Kaiser ninhydrin test to detect free amino groups of certain amino acids at the N-terminus of synthetic peptides. These results suggest the need for more careful monitoring of Fmoc synthesis reactions than previously recognized or recommended in standard protocols. We demonstrate the utility of plasma desorption mass spectrometry (PDMS) in analyzing peptide products and recommend a minimum sequence of analytical quality control including HPLC and PDMS.

Original languageEnglish (US)
Pages (from-to)19-25
Number of pages7
JournalPeptide Research
Volume4
Issue number1
StatePublished - Jan 1 1991
Externally publishedYes

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Quality Control
Quality control
Peptides
Mass spectrometry
Desorption
Mass Spectrometry
Ninhydrin
Plasmas
Amino Acids
Solid-Phase Synthesis Techniques
Monitoring
High Pressure Liquid Chromatography
Surveys and Questionnaires

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology

Cite this

A survey of potential problems and quality control in peptide synthesis by the fluorenylmethoxycarbonyl procedure. / Fontenot, J. D.; Ball, J. M.; Miller, Mark; David, C. M.; Montelaro, R. C.

In: Peptide Research, Vol. 4, No. 1, 01.01.1991, p. 19-25.

Research output: Contribution to journalArticle

Fontenot, J. D. ; Ball, J. M. ; Miller, Mark ; David, C. M. ; Montelaro, R. C. / A survey of potential problems and quality control in peptide synthesis by the fluorenylmethoxycarbonyl procedure. In: Peptide Research. 1991 ; Vol. 4, No. 1. pp. 19-25.
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