A Synthetic Peptide Analogue of a Determinant of Type II Collagen Prevents the Onset of Collagen-Induced Arthritis

Linda Myers, Edward F. Rosloniec, Jerome M. Seyer, John M. Stuart, Andrew Kang

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The immunization of genetically susceptible strains of mice with type II collagen (CII) elicits a collagen-induced arthritis that resembles rheumatoid arthritis. Our laboratory previously identified a region of CII, CII-245-270, that contains a T cell epitope that is prominent in the immune response to CII. Residues critical to the l-Aq-restricted presentation of this determinant have been characterized. To produce synthetic peptides with the potential of disrupting l-Aq-restricted Ag presentation, synthetic analogue peptides were developed that contain site-directed substitutions in critical positions. One analogue peptide was found to be an efficient competitor for binding to l-Aq and to be capable of inhibiting T cell responses in vitro. When DBA/1 mice were coimmunized with CII and the analogue peptide, the incidence and severity of arthritis were greatly reduced, concordant with the humoral immune responses to CII.

Original languageEnglish (US)
Pages (from-to)4652-4658
Number of pages7
JournalJournal of Immunology
Volume150
Issue number10
StatePublished - May 15 1993

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Experimental Arthritis
Collagen Type II
Peptides
Inbred DBA Mouse
T-Lymphocyte Epitopes
Humoral Immunity
Arthritis
Immunization
Rheumatoid Arthritis
T-Lymphocytes
Incidence

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

A Synthetic Peptide Analogue of a Determinant of Type II Collagen Prevents the Onset of Collagen-Induced Arthritis. / Myers, Linda; Rosloniec, Edward F.; Seyer, Jerome M.; Stuart, John M.; Kang, Andrew.

In: Journal of Immunology, Vol. 150, No. 10, 15.05.1993, p. 4652-4658.

Research output: Contribution to journalArticle

Myers, Linda ; Rosloniec, Edward F. ; Seyer, Jerome M. ; Stuart, John M. ; Kang, Andrew. / A Synthetic Peptide Analogue of a Determinant of Type II Collagen Prevents the Onset of Collagen-Induced Arthritis. In: Journal of Immunology. 1993 ; Vol. 150, No. 10. pp. 4652-4658.
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