A system to impose prescribed homogenous strains on cultured cells

Christopher Waters, Matthew R. Glucksberg, Eugene P. Lautenschlager, Chyh Woei Lee, Reed M. Van Matre, Richard J. Warp, Ushma Savla, Kevin E. Healy, Brian Moran, David G. Castner, Jane P. Bearinger

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

There is presently significant interest in cellular responses to physical forces, and numerous devices have been developed to apply stretch to cultured cells. Many of the early devices were limited by the heterogeneity of deformation of cells in different locations and by the high degree of anisotropy at a particular location. We have therefore developed a system to impose cyclic, large-strain, homogeneous stretch on a multiwell surface-treated silicone elastomer substrate plated with pulmonary epithelial cells. The pneumatically driven mechanism consists of four plates each with a clamp to fix one edge of the cruciform elastomer substrate. Four linear bearings set at predetermined angles between the plates ensure a constant ratio of principal strains throughout the stretch cycle. We present the design of the device and membrane shape, the surface modifications of the membrane to promote cell adhesion, predicted and experimental measurements of the strain field, and new data using cultured airway epithelial cells. We present for the first time the relationship between the magnitude of cyclic mechanical strain and the extent of wound closure and cell spreading.

Original languageEnglish (US)
Pages (from-to)1600-1610
Number of pages11
JournalJournal of applied physiology
Volume91
Issue number4
StatePublished - Oct 8 2001

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Cultured Cells
Epithelial Cells
Elastomers
Equipment Design
Silicone Elastomers
Equipment and Supplies
Membranes
Anisotropy
Cell Adhesion
Lung
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

Waters, C., Glucksberg, M. R., Lautenschlager, E. P., Lee, C. W., Van Matre, R. M., Warp, R. J., ... Bearinger, J. P. (2001). A system to impose prescribed homogenous strains on cultured cells. Journal of applied physiology, 91(4), 1600-1610.

A system to impose prescribed homogenous strains on cultured cells. / Waters, Christopher; Glucksberg, Matthew R.; Lautenschlager, Eugene P.; Lee, Chyh Woei; Van Matre, Reed M.; Warp, Richard J.; Savla, Ushma; Healy, Kevin E.; Moran, Brian; Castner, David G.; Bearinger, Jane P.

In: Journal of applied physiology, Vol. 91, No. 4, 08.10.2001, p. 1600-1610.

Research output: Contribution to journalArticle

Waters, C, Glucksberg, MR, Lautenschlager, EP, Lee, CW, Van Matre, RM, Warp, RJ, Savla, U, Healy, KE, Moran, B, Castner, DG & Bearinger, JP 2001, 'A system to impose prescribed homogenous strains on cultured cells', Journal of applied physiology, vol. 91, no. 4, pp. 1600-1610.
Waters C, Glucksberg MR, Lautenschlager EP, Lee CW, Van Matre RM, Warp RJ et al. A system to impose prescribed homogenous strains on cultured cells. Journal of applied physiology. 2001 Oct 8;91(4):1600-1610.
Waters, Christopher ; Glucksberg, Matthew R. ; Lautenschlager, Eugene P. ; Lee, Chyh Woei ; Van Matre, Reed M. ; Warp, Richard J. ; Savla, Ushma ; Healy, Kevin E. ; Moran, Brian ; Castner, David G. ; Bearinger, Jane P. / A system to impose prescribed homogenous strains on cultured cells. In: Journal of applied physiology. 2001 ; Vol. 91, No. 4. pp. 1600-1610.
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