A thermal study of cellular motility by optical time-resolved correlation

Francisco Sierra Valdez, A. J. Cisneros-Mejorado, D. P. Sánchez Herrera, J. C. Ruiz-Suárez

Research output: Contribution to journalReview article

4 Citations (Scopus)

Abstract

The study of motor properties of cells under appropriate physical-chemical conditions is a significant problem nowadays. The standard techniques presently used do not allow to evaluate neither large samples nor to control their thermodynamic conditions. In this work, we report a cell motility sensor based on an optical technique with a time-resolved correlation, adapted in a system able to study several samples simultaneously. Image correlation analysis is used to follow their temporal behavior. A wide variety of motile cells, such as archaea, bacteria, spermatozoa, and even contractile cells, can be studied using this technique. Here, we tested our technique with the study of sperm motility. In particular, both the sperm motility and its prevalence are studied under a temperature range from 0 to 37 C. We found that incubation at 10 C presents the lengthiest prevalence in motility and observed, for the first time, an interesting thermal reversibility behavior.

Original languageEnglish (US)
Article number044302
JournalReview of Scientific Instruments
Volume83
Issue number4
DOIs
StatePublished - Apr 1 2012

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locomotion
Bacteria
Hot Temperature
Sperm Motility
Thermodynamics
Sensors
cells
Archaea
spermatozoa
Temperature
Cell Movement
Spermatozoa
bacteria
thermodynamics
sensors
temperature

All Science Journal Classification (ASJC) codes

  • Instrumentation

Cite this

Sierra Valdez, F., Cisneros-Mejorado, A. J., Sánchez Herrera, D. P., & Ruiz-Suárez, J. C. (2012). A thermal study of cellular motility by optical time-resolved correlation. Review of Scientific Instruments, 83(4), [044302]. https://doi.org/10.1063/1.3700248

A thermal study of cellular motility by optical time-resolved correlation. / Sierra Valdez, Francisco; Cisneros-Mejorado, A. J.; Sánchez Herrera, D. P.; Ruiz-Suárez, J. C.

In: Review of Scientific Instruments, Vol. 83, No. 4, 044302, 01.04.2012.

Research output: Contribution to journalReview article

Sierra Valdez, F, Cisneros-Mejorado, AJ, Sánchez Herrera, DP & Ruiz-Suárez, JC 2012, 'A thermal study of cellular motility by optical time-resolved correlation', Review of Scientific Instruments, vol. 83, no. 4, 044302. https://doi.org/10.1063/1.3700248
Sierra Valdez F, Cisneros-Mejorado AJ, Sánchez Herrera DP, Ruiz-Suárez JC. A thermal study of cellular motility by optical time-resolved correlation. Review of Scientific Instruments. 2012 Apr 1;83(4). 044302. https://doi.org/10.1063/1.3700248
Sierra Valdez, Francisco ; Cisneros-Mejorado, A. J. ; Sánchez Herrera, D. P. ; Ruiz-Suárez, J. C. / A thermal study of cellular motility by optical time-resolved correlation. In: Review of Scientific Instruments. 2012 ; Vol. 83, No. 4.
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