A transcriptome survey spanning life stages and sexes of the harlequin bug, Murgantia histrionica

Michael E. Sparks, Joshua H. Rhoades, David Nelson, Daniel Kuhar, Jason Lancaster, Bryan Lehner, Dorothea Tholl, Donald C. Weber, Dawn E. Gundersen-Rindal

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The harlequin bug, Murgantia histrionica (Hahn), is an agricultural pest in the continental United States, particularly in southern states. Reliable gene sequence data are especially useful to the development of species-specific, environmentally friendly molecular biopesticides and effective biolures for this insect. Here, mRNAs were sampled from whole insects at the 2nd and 4th nymphal instars, as well as sexed adults, and sequenced using Illumina RNA-Seq technology. A global assembly of these data identified 72,540 putative unique transcripts bearing high levels of similarity to transcripts identified in other taxa, with over 99% of conserved single-copy orthologs among insects being detected. Gene ontology and protein family analyses were conducted to explore the functional potential of the harlequin bug’s gene repertoire, and phylogenetic analyses were conducted on gene families germane to xenobiotic detoxification, including glutathione S-transferases, carboxylesterases and cytochrome P450s. Genic content in harlequin bug was compared with that of the closely related invasive pest, the brown marmorated stink bug, Halyomorpha halys (Stål). Quantitative analyses of harlequin bug gene expression levels, experimentally validated using quantitative real-time PCR, identified genes differentially expressed between life stages and/or sexes.

Original languageEnglish (US)
Article number55
JournalInsects
Volume8
Issue number2
DOIs
StatePublished - Jun 1 2017

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Murgantia histrionica
transcriptome
Halyomorpha halys
gender
insects
genes
pests
carboxylesterase
biopesticides
xenobiotics
cytochromes
Southeastern United States
glutathione transferase
quantitative polymerase chain reaction
instars
RNA
nucleotide sequences
gene expression
phylogeny

All Science Journal Classification (ASJC) codes

  • Insect Science

Cite this

Sparks, M. E., Rhoades, J. H., Nelson, D., Kuhar, D., Lancaster, J., Lehner, B., ... Gundersen-Rindal, D. E. (2017). A transcriptome survey spanning life stages and sexes of the harlequin bug, Murgantia histrionica. Insects, 8(2), [55]. https://doi.org/10.3390/insects8020055

A transcriptome survey spanning life stages and sexes of the harlequin bug, Murgantia histrionica. / Sparks, Michael E.; Rhoades, Joshua H.; Nelson, David; Kuhar, Daniel; Lancaster, Jason; Lehner, Bryan; Tholl, Dorothea; Weber, Donald C.; Gundersen-Rindal, Dawn E.

In: Insects, Vol. 8, No. 2, 55, 01.06.2017.

Research output: Contribution to journalArticle

Sparks, ME, Rhoades, JH, Nelson, D, Kuhar, D, Lancaster, J, Lehner, B, Tholl, D, Weber, DC & Gundersen-Rindal, DE 2017, 'A transcriptome survey spanning life stages and sexes of the harlequin bug, Murgantia histrionica', Insects, vol. 8, no. 2, 55. https://doi.org/10.3390/insects8020055
Sparks, Michael E. ; Rhoades, Joshua H. ; Nelson, David ; Kuhar, Daniel ; Lancaster, Jason ; Lehner, Bryan ; Tholl, Dorothea ; Weber, Donald C. ; Gundersen-Rindal, Dawn E. / A transcriptome survey spanning life stages and sexes of the harlequin bug, Murgantia histrionica. In: Insects. 2017 ; Vol. 8, No. 2.
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