A transgenic mouse class-III βtubulin reporter using yellow fluorescent protein

Li Liu, Eldon E. Geisert, Anthony Frankfurter, Anthony J. Spano, Xue Jiang Chloe, Junming Yue, Ioannis Dragatsis, Dan Goldowitz

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A yellow fluorescence protein (YFP) reporter construct was cloned downstream of the β-tubulin III promoter and injected to produce two founder lines of transgenic mice. YFP expression was observed in many regions of the developing peripheral and central nervous system. YFP expression was first observed in the peripheral and central nervous system as early as embryonic day 9.0. There was a dramatic increase in the number of neuronal systems expressing YFP through P0. Then as the animals reached adult age, the expression levels decreased, but many neurons still show YFP expression, notably in regions of the brain undergoing adult neurogenesis, i.e., the rostral migratory stream and subgranular layer of the dentate gyrus. This reporter-based staining was compared with anti-class-III β-tubulin immunocytochemistry and shown to closely parallel the expression of the endogenous protein. These transgenic lines should provide unique models to study in vivo and in vitro neurodevelopment.

Original languageEnglish (US)
Pages (from-to)560-569
Number of pages10
JournalGenesis
Volume45
Issue number9
DOIs
StatePublished - Sep 1 2007

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Tubulin
Transgenic Mice
Fluorescence
Peripheral Nervous System
Proteins
Tubulin Modulators
Central Nervous System
Myelin P0 Protein
Neurogenesis
Dentate Gyrus
Immunohistochemistry
Staining and Labeling
Neurons
Brain

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Liu, L., Geisert, E. E., Frankfurter, A., Spano, A. J., Chloe, X. J., Yue, J., ... Goldowitz, D. (2007). A transgenic mouse class-III βtubulin reporter using yellow fluorescent protein. Genesis, 45(9), 560-569. https://doi.org/10.1002/dvg.20325

A transgenic mouse class-III βtubulin reporter using yellow fluorescent protein. / Liu, Li; Geisert, Eldon E.; Frankfurter, Anthony; Spano, Anthony J.; Chloe, Xue Jiang; Yue, Junming; Dragatsis, Ioannis; Goldowitz, Dan.

In: Genesis, Vol. 45, No. 9, 01.09.2007, p. 560-569.

Research output: Contribution to journalArticle

Liu, L, Geisert, EE, Frankfurter, A, Spano, AJ, Chloe, XJ, Yue, J, Dragatsis, I & Goldowitz, D 2007, 'A transgenic mouse class-III βtubulin reporter using yellow fluorescent protein', Genesis, vol. 45, no. 9, pp. 560-569. https://doi.org/10.1002/dvg.20325
Liu L, Geisert EE, Frankfurter A, Spano AJ, Chloe XJ, Yue J et al. A transgenic mouse class-III βtubulin reporter using yellow fluorescent protein. Genesis. 2007 Sep 1;45(9):560-569. https://doi.org/10.1002/dvg.20325
Liu, Li ; Geisert, Eldon E. ; Frankfurter, Anthony ; Spano, Anthony J. ; Chloe, Xue Jiang ; Yue, Junming ; Dragatsis, Ioannis ; Goldowitz, Dan. / A transgenic mouse class-III βtubulin reporter using yellow fluorescent protein. In: Genesis. 2007 ; Vol. 45, No. 9. pp. 560-569.
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