A variant hepatitis E isolated from a hepatitis E patient with negative anti-HEV

Kui Li, Hui Zhuang, Youchun Wang

Research output: Contribution to journalArticle

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Abstract

Objective To investigate genotypes of hepatitis E viruses isolated from hepatitis E patients with negative and-HEV. Methods Acute-phase sera of 22 hepatitis patients with negative anti-HAV IgM, HBsAg, anti-HBc IgM, anti-HCV and anti-HEV were detected for HEV RNA using a RT-nPCR based on a set of degenerate primers derived from open reading frame 2 of HEV. The positive PCR products were directly sequenced. Results Among 22 acute hepatitis patients, 9(40.9%) were HEV RNA positive. Six PCR products of 9 sera were directly sequenced. Five of them shared the same subtype with HEV Chinese strain (L25595), with the nucleotide identity of 98.0% and the amino acid identity of 99.0%. The remainder, designated HEV LZ-105, was a variant of HEV. The nucleotide and amino acid homology of HEV LZ-105 with HEV(China), HEV(Burma), HEV( Mexico), and the newly identified HEV US-1 and swine HEV was 77.4%, 78.1%, 74.3%, 77.4%, 77.1% and 91.0%,91.0%,89.0%,93.0%,93.0%, respectively. Conclusion A variant of HEV was isolated from a hepatitis E patient with negative anti-HEV.

Original languageEnglish (US)
Pages (from-to)164-166
Number of pages3
JournalChinese Journal of Microbiology and Immunology
Volume20
Issue number2
StatePublished - Mar 2000
Externally publishedYes

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Hepatitis E
Hepatitis
Nucleotides
RNA
Hepatitis A Antibodies
Myanmar
Hepatitis E virus
Amino Acids
Polymerase Chain Reaction
Hepatitis B Surface Antigens
Mexico
Serum
Open Reading Frames
China
Swine
Genotype
anti-IgM

All Science Journal Classification (ASJC) codes

  • Immunology and Microbiology(all)
  • Microbiology (medical)

Cite this

A variant hepatitis E isolated from a hepatitis E patient with negative anti-HEV. / Li, Kui; Zhuang, Hui; Wang, Youchun.

In: Chinese Journal of Microbiology and Immunology, Vol. 20, No. 2, 03.2000, p. 164-166.

Research output: Contribution to journalArticle

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abstract = "Objective To investigate genotypes of hepatitis E viruses isolated from hepatitis E patients with negative and-HEV. Methods Acute-phase sera of 22 hepatitis patients with negative anti-HAV IgM, HBsAg, anti-HBc IgM, anti-HCV and anti-HEV were detected for HEV RNA using a RT-nPCR based on a set of degenerate primers derived from open reading frame 2 of HEV. The positive PCR products were directly sequenced. Results Among 22 acute hepatitis patients, 9(40.9{\%}) were HEV RNA positive. Six PCR products of 9 sera were directly sequenced. Five of them shared the same subtype with HEV Chinese strain (L25595), with the nucleotide identity of 98.0{\%} and the amino acid identity of 99.0{\%}. The remainder, designated HEV LZ-105, was a variant of HEV. The nucleotide and amino acid homology of HEV LZ-105 with HEV(China), HEV(Burma), HEV( Mexico), and the newly identified HEV US-1 and swine HEV was 77.4{\%}, 78.1{\%}, 74.3{\%}, 77.4{\%}, 77.1{\%} and 91.0{\%},91.0{\%},89.0{\%},93.0{\%},93.0{\%}, respectively. Conclusion A variant of HEV was isolated from a hepatitis E patient with negative anti-HEV.",
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N2 - Objective To investigate genotypes of hepatitis E viruses isolated from hepatitis E patients with negative and-HEV. Methods Acute-phase sera of 22 hepatitis patients with negative anti-HAV IgM, HBsAg, anti-HBc IgM, anti-HCV and anti-HEV were detected for HEV RNA using a RT-nPCR based on a set of degenerate primers derived from open reading frame 2 of HEV. The positive PCR products were directly sequenced. Results Among 22 acute hepatitis patients, 9(40.9%) were HEV RNA positive. Six PCR products of 9 sera were directly sequenced. Five of them shared the same subtype with HEV Chinese strain (L25595), with the nucleotide identity of 98.0% and the amino acid identity of 99.0%. The remainder, designated HEV LZ-105, was a variant of HEV. The nucleotide and amino acid homology of HEV LZ-105 with HEV(China), HEV(Burma), HEV( Mexico), and the newly identified HEV US-1 and swine HEV was 77.4%, 78.1%, 74.3%, 77.4%, 77.1% and 91.0%,91.0%,89.0%,93.0%,93.0%, respectively. Conclusion A variant of HEV was isolated from a hepatitis E patient with negative anti-HEV.

AB - Objective To investigate genotypes of hepatitis E viruses isolated from hepatitis E patients with negative and-HEV. Methods Acute-phase sera of 22 hepatitis patients with negative anti-HAV IgM, HBsAg, anti-HBc IgM, anti-HCV and anti-HEV were detected for HEV RNA using a RT-nPCR based on a set of degenerate primers derived from open reading frame 2 of HEV. The positive PCR products were directly sequenced. Results Among 22 acute hepatitis patients, 9(40.9%) were HEV RNA positive. Six PCR products of 9 sera were directly sequenced. Five of them shared the same subtype with HEV Chinese strain (L25595), with the nucleotide identity of 98.0% and the amino acid identity of 99.0%. The remainder, designated HEV LZ-105, was a variant of HEV. The nucleotide and amino acid homology of HEV LZ-105 with HEV(China), HEV(Burma), HEV( Mexico), and the newly identified HEV US-1 and swine HEV was 77.4%, 78.1%, 74.3%, 77.4%, 77.1% and 91.0%,91.0%,89.0%,93.0%,93.0%, respectively. Conclusion A variant of HEV was isolated from a hepatitis E patient with negative anti-HEV.

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