A yeast protein phosphatase related to the vaccinia virus VH1 phosphatase is induced by nitrogen starvation

K. Guan, D. J. Hakes, Y. Wang, H. D. Park, Terrance Cooper, J. E. Dixon

Research output: Contribution to journalArticle

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Abstract

A phosphatase related to the vaccinia virus VH1 phosphatase has been cloned from Saccharomyces cerevisiae. The yeast phosphatase is related to the Schizosaccharomyces pombe cdc25 gene product and to a protein encoded by a mammalian open reading frame known as 3CH134, which is an immediate early gene responding to serum stimulation. The phosphatase activity of the yeast gene product appears to be restricted to the hydrolysis of phosphotyrosine- containing substrates, whereas the vaccinia phosphatase hydrolyzes both phosphoserine- and phosphotyrosine-containing substrates. The mRNA encoding the yeast phosphatase is dramatically induced by nitrogen starvation. Inactivation of the yeast phosphatase gene results in a decrease in growth rate.

Original languageEnglish (US)
Pages (from-to)12175-12179
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number24
DOIs
StatePublished - Dec 1 1992

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Fungal Proteins
Vaccinia virus
Phosphoprotein Phosphatases
Starvation
Phosphoric Monoester Hydrolases
Nitrogen
Yeasts
Phosphotyrosine
Genes
Phosphoserine
Vaccinia
Immediate-Early Genes
Schizosaccharomyces
Open Reading Frames
Saccharomyces cerevisiae
Hydrolysis
Messenger RNA
Growth
Serum

All Science Journal Classification (ASJC) codes

  • General

Cite this

A yeast protein phosphatase related to the vaccinia virus VH1 phosphatase is induced by nitrogen starvation. / Guan, K.; Hakes, D. J.; Wang, Y.; Park, H. D.; Cooper, Terrance; Dixon, J. E.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 24, 01.12.1992, p. 12175-12179.

Research output: Contribution to journalArticle

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