Abnormalities caused by carbohydrate alterations in Iβ6-N- acetylglucosaminyltransferase-deficient mice

Guoyun Chen, Hisako Muramatsu, Mineo Kondo, Nobuyuki Kurosawa, Yozo Miyake, Naoki Takeda, Takashi Muramatsu

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Iβ6-N-acetylglucosaminyltransferase (IGnT) catalyzes the branching of poly-N-acetyllactosamine carbohydrate chains. In both humans and mice, three spliced forms of IGnT have been identified, and a common exon is present in all of them. We generated mice deficient in the common exon to understand the physiological function of poly-N-acetyllactosamine branching. IGnT activity was abolished in the stomach, kidney, bone marrow, and cerebellum of the deficient mice, while a low level of the activity persisted in the small intestine. Immunohistochemical analysis confirmed the loss of I antigen from the lung, stomach, and kidney. The deficient mice had reduced spontaneous locomotive activity. The number of peripheral blood lymphocytes was also reduced and renal function decreased in the deficient mice. Furthermore, in aged mice, vacuolization occurred in the kidney, and epidermoid cysts were frequently formed. However, cataracts did not develop earlier in the deficient mice. Decreased levels of lysosomal proteins, LAMP-2 and synaptotagmin VII, were found in the kidney of the deficient mice and correlated with renal abnormalities.

Original languageEnglish (US)
Pages (from-to)7828-7838
Number of pages11
JournalMolecular and cellular biology
Volume25
Issue number17
DOIs
StatePublished - Sep 1 2005

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Carbohydrates
Kidney
Exons
Stomach
Synaptotagmins
Epidermal Cyst
N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase
Cataract
Cerebellum
Small Intestine
Bone Marrow
Lymphocytes
Lung

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Abnormalities caused by carbohydrate alterations in Iβ6-N- acetylglucosaminyltransferase-deficient mice. / Chen, Guoyun; Muramatsu, Hisako; Kondo, Mineo; Kurosawa, Nobuyuki; Miyake, Yozo; Takeda, Naoki; Muramatsu, Takashi.

In: Molecular and cellular biology, Vol. 25, No. 17, 01.09.2005, p. 7828-7838.

Research output: Contribution to journalArticle

Chen, Guoyun ; Muramatsu, Hisako ; Kondo, Mineo ; Kurosawa, Nobuyuki ; Miyake, Yozo ; Takeda, Naoki ; Muramatsu, Takashi. / Abnormalities caused by carbohydrate alterations in Iβ6-N- acetylglucosaminyltransferase-deficient mice. In: Molecular and cellular biology. 2005 ; Vol. 25, No. 17. pp. 7828-7838.
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