Abscisic acid and stress signals induce Viviparous1 expression in seed and vegetative tissues of maize

Xueyuan Cao, Liliana M. Costa, Corinne Biderre-Petit, Bouchab Kbhaya, Nrisingha Dey, Pascual Perez, Donald R. McCarty, Jose F. Gutierrez-Marcos, Philip W. Becraft

Research output: Contribution to journalArticle

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Abstract

Viviparous1 (Vp1) encodes a B3 domain-containing transcription factor that is a key regulator of seed maturation in maize (Zea mays). However, the mechanisms of Vp1 regulation are not well understood. To examine physiological factors that may regulate Vp1 expression, transcript levels were monitored in maturing embryos placed in culture under different conditions. Expression of Vp1 decreased after culture in hormone-free medium, but was induced by salinity or osmotic stress. Application of exogenous abscisic acid (ABA) also induced transcript levels within 1 h in a dose-dependent manner. The Vp1 promoter fused to β-glucuronidase or green fluorescent protein reproduced the endogenous Vp1 expression patterns in transgenic maize plants and also revealed previously unknown expression domains of Vp1. The Vp1 promoter is active in the embryo and aleurone cells of developing seeds and, upon drought stress, was also found in phloem cells of vegetative tissues, including cobs, leaves, and stems. Sequence analysis of the Vp1 promoter identified a potential ABA-responsive complex, consisting of an ACGT-containing ABA response element (ABRE) and a coupling element 1-like motif. Electrophoretic mobility shift assay confirmed that the ABRE and putative coupling element 1 components specifically bound proteins in embryo nuclear protein extracts. Treatment of embryos in hormone-free Murashige and Skoog medium blocked the ABRE-protein interaction, whereas exogenous ABA or mannitol treatment restored this interaction. Our data support a model for a VP1-dependent positive feedback mechanism regulating Vp1 expression during seed maturation.

Original languageEnglish (US)
Pages (from-to)720-731
Number of pages12
JournalPlant physiology
Volume143
Issue number2
DOIs
StatePublished - Feb 1 2007

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Abscisic Acid
Zea mays
abscisic acid
Seeds
Response Elements
Embryonic Structures
corn
response elements
seeds
embryo (plant)
seed maturation
promoter regions
Hormones
Phloem
Genetically Modified Plants
Droughts
Glucuronidase
Osmotic Pressure
Salinity
Electrophoretic Mobility Shift Assay

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

Cao, X., Costa, L. M., Biderre-Petit, C., Kbhaya, B., Dey, N., Perez, P., ... Becraft, P. W. (2007). Abscisic acid and stress signals induce Viviparous1 expression in seed and vegetative tissues of maize. Plant physiology, 143(2), 720-731. https://doi.org/10.1104/pp.106.091454

Abscisic acid and stress signals induce Viviparous1 expression in seed and vegetative tissues of maize. / Cao, Xueyuan; Costa, Liliana M.; Biderre-Petit, Corinne; Kbhaya, Bouchab; Dey, Nrisingha; Perez, Pascual; McCarty, Donald R.; Gutierrez-Marcos, Jose F.; Becraft, Philip W.

In: Plant physiology, Vol. 143, No. 2, 01.02.2007, p. 720-731.

Research output: Contribution to journalArticle

Cao, X, Costa, LM, Biderre-Petit, C, Kbhaya, B, Dey, N, Perez, P, McCarty, DR, Gutierrez-Marcos, JF & Becraft, PW 2007, 'Abscisic acid and stress signals induce Viviparous1 expression in seed and vegetative tissues of maize', Plant physiology, vol. 143, no. 2, pp. 720-731. https://doi.org/10.1104/pp.106.091454
Cao, Xueyuan ; Costa, Liliana M. ; Biderre-Petit, Corinne ; Kbhaya, Bouchab ; Dey, Nrisingha ; Perez, Pascual ; McCarty, Donald R. ; Gutierrez-Marcos, Jose F. ; Becraft, Philip W. / Abscisic acid and stress signals induce Viviparous1 expression in seed and vegetative tissues of maize. In: Plant physiology. 2007 ; Vol. 143, No. 2. pp. 720-731.
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