Absence of brain spectrin( 240 235) in dendrites of mammalian brain

Ian S. Zagon, Steven Goodman

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Spectrin is a major cytoskeletal component of the brain. At least 3 distinct spectrin subtypes are found in mammalian brain: brain spectrin( 240 235) which is confined mainly to axons, brain spectrin( 240 235E) which is localized largely in neural cell bodies and dendrites, and brain spectrin( 240 235A) which is associated only with astrocytes. Recently, Ivy et al. (5) reported that brain spectrin ( 240 235) was located in dendrites when tissues were fixed with 4% paraformaldehyde. To evaluate this matter further, rat cerebellar cortex prepared with and without aldehydes was stained with antibodies to brain spectrin( 240 235) and examined using peroxidase (4-chloro-1-naphthol or avidin-biotin) or rhodamine to visualize the primary antibody. The preparations (10 μm and 40 μm sections) showed that brain spectrin( 240 235) resided largely in axons with occasional staining of neuronal soma (Purkinje cells), but was not observed in dendrites. These results confirm earlier reports [e.g., (10,12)] showing the discrete compartmentalization of brain spectrin( 240 235) in axons and cell bodies. Brain Spectrin Fodrin Cytoskeleton Neuroanatomy Immunocytochemistry.

Original languageEnglish (US)
Pages (from-to)19-24
Number of pages6
JournalBrain Research Bulletin
Volume23
Issue number1-2
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Spectrin
Dendrites
Brain
Axons
Neuroanatomy
Cerebellar Cortex
Rhodamines
Avidin
Antibodies
Purkinje Cells
Carisoprodol
Biotin
Cytoskeleton
Aldehydes
Astrocytes
Peroxidase
Immunohistochemistry
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Absence of brain spectrin( 240 235) in dendrites of mammalian brain. / Zagon, Ian S.; Goodman, Steven.

In: Brain Research Bulletin, Vol. 23, No. 1-2, 01.01.1989, p. 19-24.

Research output: Contribution to journalArticle

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